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- W2012167747 abstract "The quontitation of RNA in tissue homogenates by amplifying the product of reverse transcription (RT-PCR) is sufficiently sensitive to detect molecules in the range of 10−110−2 amole. We describe here the steps we believe necessary to validate a protocal that used a DNA competitor and visualization of the amplification products by ethidium bromide staining. The procedure was designed to quantitate one of the tissue specific transcripts of the Dopa decarboxylase gene (Ddc) in Drosophila. We demonstrate that the amount of epidermal Ddc transcript is much lower at pupariation in several mutants of the Broad-Complex, one of the primary response loci of the moulting hormone, ecdysone. The mutant effects were allele specific and the molecular basis of one of these alleles is known. This implicates a particular family of the zinc finger proteins encoded by the locus in the hormone dependent induction of Ddc expression." @default.
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- W2012167747 date "1995-01-01" @default.
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- W2012167747 title "Epidermal cell-specific quantitation of dopa decarboxylase mRNA inDrosophila by competitive RT-PCR: An effect ofBroad-Complex mutants" @default.
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- W2012167747 doi "https://doi.org/10.1002/dvg.1020160111" @default.
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