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- W2012172841 abstract "From the serum of Pangasianodon hypophthalmus, two proteins were isolated by affinity chromatography on Sepharose and phosphorylcholine–Sepharose. Their binding on the affinity matrices critically depends on the presence of Ca2+ ions. N-terminal sequencing and sequencing of internal tryptic peptides identified the proteins as pentraxins and from their binding properties they are identified as SAP (serum amyloid P component) and CRP (C-reactive protein). Per ml serum, 36 μg SAP and 56 μg CRP was purified. Upon gel filtration, both the SAP and CRP elute as trimers of respectively 24 kDa and 28 kDa subunits. Both proteins are devoid of inter-chain disulfide bonds. Both SAP and CRP are glycosylated and agglutinate rabbit erythrocytes and pathogenic bacteria Edwardsiella ictaluri and Aeromonas hydrophila, but not Micrococcus lysodeikticus or Escherichia coli. Haemagglutination of SAP and CRP is inhibited by galactose (MIC = 1 mM) and by phosphorylcholine (MIC = 1–2 mM), respectively. Circular dichroism studies revealed that antiparallel β-pleated sheets are dominating the secondary structure. Upon removing the Ca2+ ions by EDTA, slight structural changes are observed by CD spectroscopy in the near-UV region. Immunodiffusion shows that P. hypophthalmus SAP and CRP do not cross-react." @default.
- W2012172841 created "2016-06-24" @default.
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- W2012172841 date "2010-05-01" @default.
- W2012172841 modified "2023-10-02" @default.
- W2012172841 title "Isolation and characterization of SAP and CRP, two pentraxins from Pangasianodon (Pangasius) hypophthalmus" @default.
- W2012172841 cites W1515009092 @default.
- W2012172841 cites W1526570214 @default.
- W2012172841 cites W1530950071 @default.
- W2012172841 cites W1597973709 @default.
- W2012172841 cites W1679413993 @default.
- W2012172841 cites W1796487270 @default.
- W2012172841 cites W1958031853 @default.
- W2012172841 cites W1964447469 @default.
- W2012172841 cites W1969576580 @default.
- W2012172841 cites W1975085160 @default.
- W2012172841 cites W1977358487 @default.
- W2012172841 cites W1979783822 @default.
- W2012172841 cites W1980251674 @default.
- W2012172841 cites W1980851748 @default.
- W2012172841 cites W1982988924 @default.
- W2012172841 cites W1984965187 @default.
- W2012172841 cites W1986699235 @default.
- W2012172841 cites W1988043449 @default.
- W2012172841 cites W1988177816 @default.
- W2012172841 cites W1993917313 @default.
- W2012172841 cites W1994906352 @default.
- W2012172841 cites W1996120330 @default.
- W2012172841 cites W1996805644 @default.
- W2012172841 cites W1998440525 @default.
- W2012172841 cites W1998905926 @default.
- W2012172841 cites W1999599945 @default.
- W2012172841 cites W2002706411 @default.
- W2012172841 cites W2005713983 @default.
- W2012172841 cites W2005753753 @default.
- W2012172841 cites W2005879882 @default.
- W2012172841 cites W2015701191 @default.
- W2012172841 cites W2023746903 @default.
- W2012172841 cites W2024886564 @default.
- W2012172841 cites W2028643532 @default.
- W2012172841 cites W2038318534 @default.
- W2012172841 cites W2038558326 @default.
- W2012172841 cites W2039115511 @default.
- W2012172841 cites W2040849321 @default.
- W2012172841 cites W2040983174 @default.
- W2012172841 cites W2042970707 @default.
- W2012172841 cites W2044703430 @default.
- W2012172841 cites W2047522139 @default.
- W2012172841 cites W2047761047 @default.
- W2012172841 cites W2048985212 @default.
- W2012172841 cites W2049835391 @default.
- W2012172841 cites W2053706055 @default.
- W2012172841 cites W2056026742 @default.
- W2012172841 cites W2063572291 @default.
- W2012172841 cites W2071904585 @default.
- W2012172841 cites W2074112745 @default.
- W2012172841 cites W2079522334 @default.
- W2012172841 cites W2079823337 @default.
- W2012172841 cites W2083509428 @default.
- W2012172841 cites W2085597672 @default.
- W2012172841 cites W2087151227 @default.
- W2012172841 cites W2089720783 @default.
- W2012172841 cites W2091366683 @default.
- W2012172841 cites W2092233764 @default.
- W2012172841 cites W2101108802 @default.
- W2012172841 cites W2105779796 @default.
- W2012172841 cites W2106882534 @default.
- W2012172841 cites W2108480363 @default.
- W2012172841 cites W2109280121 @default.
- W2012172841 cites W2114847951 @default.
- W2012172841 cites W2135659747 @default.
- W2012172841 cites W2144648204 @default.
- W2012172841 cites W2148958130 @default.
- W2012172841 cites W2149853992 @default.
- W2012172841 cites W2154746915 @default.
- W2012172841 cites W2157720962 @default.
- W2012172841 cites W2158714788 @default.
- W2012172841 cites W2332180392 @default.
- W2012172841 cites W2333303210 @default.
- W2012172841 cites W2469156062 @default.
- W2012172841 cites W2913717555 @default.
- W2012172841 cites W305088489 @default.
- W2012172841 cites W329437070 @default.
- W2012172841 cites W337161024 @default.
- W2012172841 cites W2063968553 @default.
- W2012172841 doi "https://doi.org/10.1016/j.fsi.2010.01.007" @default.
- W2012172841 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20109557" @default.
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