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- W2012173660 abstract "For critical histological investigations, tissue fixation is sometimes carried out in formaldehyde freshly prepared from paraformaldehyde by heating. The purity of formaldehyde produced in this way is superior to that of commercial stock solutions. We studied the stability of freshly prepared formaldehyde solutions by determination of pH and titration of acid, which reflect the formation of formic acid. It was found that very small amounts of acid are produced during the heating of paraformaldehyde. Prolonged heating or storage of freshly prepared formaldehyde for up to 8 days did not significantly increase the amount of acid. It was also found that heating of the paraformaldehyde is not necessary, since depolymerization may take place at room temperature. We conclude that formaldehyde prepared from paraformaldehyde remains stable for considerable periods of time, and it is therefore unnecessary to prepare it immediately prior to fixation. Also, in many cases, buffering of the fixative may be omitted, since only minor changes in the pH occur during fixation." @default.
- W2012173660 created "2016-06-24" @default.
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- W2012173660 date "2000-01-01" @default.
- W2012173660 modified "2023-10-14" @default.
- W2012173660 title "Formaldehyde prepared from paraformaldehyde is stable" @default.
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- W2012173660 doi "https://doi.org/10.3109/10520290009047980" @default.
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