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- W2012174194 abstract "We have performed a phenotypic and molecular analysis of monoclonal TCR gamma/delta T-cell lines derived from jejunal and colonic biopsies of healthy individuals. Flow cytometric analysis employing a panel of 24 monoclonal antibodies (MoAbs) demonstrated that intestinal TCR gamma/delta intraepithelial lymphocytes (IEL) constitute a phenotypically heterogeneous population. Nucleotide sequence analysis of expressed TCR delta variable (V) regions revealed the dominant utilization of the V delta 2 and D delta 3 gene segments and frequent rearrangement of J delta 3. IEL V delta regions displayed extensive junctional diversity as a result of N and P insertion and the utilization of D delta 3 in all three reading frames. The results demonstrate that intestinal TCR gamma/delta T cells from healthy individuals constitute a phenotypically heterogeneous population expressing V delta regions that differ from their systemic counterparts." @default.
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- W2012174194 date "1993-09-01" @default.
- W2012174194 modified "2023-09-25" @default.
- W2012174194 title "Phenotypic and Molecular Characterization of Human Monoclonal TCRgamma/deltaT-Cell Lines from Jejunum and Colon of Healthy Individuals" @default.
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- W2012174194 doi "https://doi.org/10.1111/j.1365-3083.1993.tb01721.x" @default.
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