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- W2012174705 abstract "Free fatty acids (FFAs) in fat are important indicator of raw milk quality. Result reliability of FFAs is important. Aim was to verify MIR-FT (mid infrared spectroscopy with Fourier's transformations) method for its calibration to determine FFAs, time stability of MIR-FT FFA calibration and calibration levelling in laboratory networks. Reference (RE) milk samples (1 set = 8) were prepared according to CSN 57 0533 (FFAs in mmol.100g-1 of fat). MIR-FT instruments were: 1 LactoScope FTIR (DE); 2 Bentley FTS (BE); 2 MilkoScan FT 6000 (FO). 3 calibrations of MIR-FT (5) in 3 laboratories were performed. Bulk milk samples came from 4 herds and 2 breeds. These 4 samples were used for calibration in native and modified form. Modification increased FFAs by cca 100%. Calibration set had 8 samples. 1 between calibration interval was checked monthly by proficiency testing (PT). PT set had 10 samples. 5 samples were with native milk and 5 had modified fat content, lower and higher. Maximal value of difference variability for calibration quality validation is x (sd of difference MIR-FT and RE) plus 1.64 multiple of sd (on 95% level), 1.0613 mmol.100g-1. Mean validation correlation coefficient (r) between MIR-FT and RE results was 0.802 ± 0.082 (P 0.802). Example PT with 10 and 5 milk samples had similar results of r 0.887 and 0.953 (P < 0.001 and P < 0.05). There is possibility to construct a levelling programme for calibrated instruments. Some equation between PT reference and instrumental values could correct MIR-FT results for their better comparability." @default.
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- W2012174705 date "2013-11-24" @default.
- W2012174705 modified "2023-10-16" @default.
- W2012174705 title "Evaluation of development in indirect determination of milk fat free fatty acids in Czech Republic" @default.
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- W2012174705 doi "https://doi.org/10.11118/actaun201361061669" @default.
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