FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012174837> ?p ?o ?g. }

• W2012174837 endingPage "17" @default.
• W2012174837 startingPage "10" @default.
• W2012174837 abstract "The neuronal nicotinic acetylcholine receptor alpha4 and beta2 subunits expressed in heterologous expression systems assemble into at least two distinct subunit stoichiometries of alpha4beta2 receptor. The (alpha4)2(beta2)3 stoichiometry is about 100-fold more sensitive to acetylcholine than the (alpha4)3(beta2)2 stoichiometry. In order to investigate if agonists in general distinguish high- and low-affinity alpha4beta2 nicotinic acetylcholine receptors, we have expressed human alpha4 and beta2 nicotinic acetylcholine receptor subunits in two different expression systems. The relative amounts of alpha4beta2 nicotinic acetylcholine receptors with high- and low-affinity for acetylcholine were manipulated by (a) injecting the subunit cDNAs at different alpha:beta ratios into Xenopus oocytes and (b) by culturing HEK-293 cells stably expressing alpha4beta2 nicotinic acetylcholine receptors overnight at different temperatures. The sensitivities of the alpha4beta2 nicotinic acetylcholine receptors to the agonists acetylcholine, 5-I A-85380, and TC-2559 were investigated using the voltage-clamp technique on Xenopus oocytes and using a fluorescent imaging plate reader to measure calcium responses from HEK-293 cells. Like acetylcholine, 5-I A-85380 produced biphasic concentration-response curves and the high-affinity component became larger when the cells were manipulated to produce a greater proportion of (alpha4)2(beta2)3 nicotinic acetylcholine receptors. Interestingly, under all circumstances, TC-2559 produced monophasic concentration-response curves. In oocytes injected with alpha4 and beta2 subunits in the 1:1 ratio the maximum effect of TC-2559 was 28% of that of acetylcholine. The EC50 for TC-2559 was not changed when oocytes were manipulated to express exclusively (alpha4)2(beta2)3 nicotinic acetylcholine receptors, however, the maximum effect of TC-2559 was dramatically enhanced. These results suggest that TC-2559 is a selective agonist of the (alpha4)2(beta2)3 nicotinic acetylcholine receptor stoichiometry." @default.
• W2012174837 created "2016-06-24" @default.
• W2012174837 creator A5008006905 @default.
• W2012174837 creator A5030560026 @default.
• W2012174837 creator A5037506999 @default.
• W2012174837 creator A5043250344 @default.
• W2012174837 creator A5046207186 @default.
• W2012174837 creator A5069419392 @default.
• W2012174837 creator A5088361219 @default.
• W2012174837 date "2006-06-01" @default.
• W2012174837 modified "2023-10-05" @default.
• W2012174837 title "5-I A-85380 and TC-2559 differentially activate heterologously expressed α4β2 nicotinic receptors" @default.
• W2012174837 cites W1503583964 @default.
• W2012174837 cites W1520253501 @default.
• W2012174837 cites W1531561158 @default.
• W2012174837 cites W1538286609 @default.
• W2012174837 cites W1799536240 @default.
• W2012174837 cites W1959145322 @default.
• W2012174837 cites W1974789469 @default.
• W2012174837 cites W1980333967 @default.
• W2012174837 cites W1995625884 @default.
• W2012174837 cites W2007226311 @default.
• W2012174837 cites W2011620653 @default.
• W2012174837 cites W2013341464 @default.
• W2012174837 cites W2019931874 @default.
• W2012174837 cites W2031173955 @default.
• W2012174837 cites W2037097116 @default.
• W2012174837 cites W2039975064 @default.
• W2012174837 cites W2049084706 @default.
• W2012174837 cites W2053266420 @default.
• W2012174837 cites W2097682864 @default.
• W2012174837 cites W2115872169 @default.
• W2012174837 cites W2133636515 @default.
• W2012174837 cites W2134231639 @default.
• W2012174837 cites W2147835957 @default.
• W2012174837 cites W2148168931 @default.
• W2012174837 cites W2153609942 @default.
• W2012174837 cites W2158847001 @default.
• W2012174837 cites W2337946333 @default.
• W2012174837 cites W4317639052 @default.
• W2012174837 doi "https://doi.org/10.1016/j.ejphar.2006.03.077" @default.
• W2012174837 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16674940" @default.
• W2012174837 hasPublicationYear "2006" @default.
• W2012174837 type Work @default.
• W2012174837 sameAs 2012174837 @default.
• W2012174837 citedByCount "39" @default.
• W2012174837 countsByYear W20121748372012 @default.
• W2012174837 countsByYear W20121748372013 @default.
• W2012174837 countsByYear W20121748372014 @default.
• W2012174837 countsByYear W20121748372015 @default.
• W2012174837 countsByYear W20121748372016 @default.
• W2012174837 countsByYear W20121748372017 @default.
• W2012174837 countsByYear W20121748372018 @default.
• W2012174837 countsByYear W20121748372020 @default.
• W2012174837 countsByYear W20121748372021 @default.
• W2012174837 countsByYear W20121748372022 @default.
• W2012174837 countsByYear W20121748372023 @default.
• W2012174837 crossrefType "journal-article" @default.
• W2012174837 hasAuthorship W2012174837A5008006905 @default.
• W2012174837 hasAuthorship W2012174837A5030560026 @default.
• W2012174837 hasAuthorship W2012174837A5037506999 @default.
• W2012174837 hasAuthorship W2012174837A5043250344 @default.
• W2012174837 hasAuthorship W2012174837A5046207186 @default.
• W2012174837 hasAuthorship W2012174837A5069419392 @default.
• W2012174837 hasAuthorship W2012174837A5088361219 @default.
• W2012174837 hasConcept C104317684 @default.
• W2012174837 hasConcept C116289061 @default.
• W2012174837 hasConcept C12554922 @default.
• W2012174837 hasConcept C170493617 @default.
• W2012174837 hasConcept C185592680 @default.
• W2012174837 hasConcept C188987157 @default.
• W2012174837 hasConcept C19876734 @default.
• W2012174837 hasConcept C2775910092 @default.
• W2012174837 hasConcept C2779535977 @default.
• W2012174837 hasConcept C2779570518 @default.
• W2012174837 hasConcept C45855136 @default.
• W2012174837 hasConcept C51927110 @default.
• W2012174837 hasConcept C55493867 @default.
• W2012174837 hasConcept C64615621 @default.
• W2012174837 hasConcept C80161118 @default.
• W2012174837 hasConcept C86803240 @default.
• W2012174837 hasConcept C98274493 @default.
• W2012174837 hasConceptScore W2012174837C104317684 @default.
• W2012174837 hasConceptScore W2012174837C116289061 @default.
• W2012174837 hasConceptScore W2012174837C12554922 @default.
• W2012174837 hasConceptScore W2012174837C170493617 @default.
• W2012174837 hasConceptScore W2012174837C185592680 @default.
• W2012174837 hasConceptScore W2012174837C188987157 @default.
• W2012174837 hasConceptScore W2012174837C19876734 @default.
• W2012174837 hasConceptScore W2012174837C2775910092 @default.
• W2012174837 hasConceptScore W2012174837C2779535977 @default.
• W2012174837 hasConceptScore W2012174837C2779570518 @default.
• W2012174837 hasConceptScore W2012174837C45855136 @default.
• W2012174837 hasConceptScore W2012174837C51927110 @default.
• W2012174837 hasConceptScore W2012174837C55493867 @default.
• W2012174837 hasConceptScore W2012174837C64615621 @default.
• W2012174837 hasConceptScore W2012174837C80161118 @default.
• W2012174837 hasConceptScore W2012174837C86803240 @default.

• next