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• W2012175205 abstract "The emerging field of synthetic biology builds gene circuits for scientific, industrial and therapeutic needs. Adaptability of synthetic gene circuits across different organisms could enable a synthetic biology pipeline, where circuits are designed in silico, characterized in microbes and reimplemented in mammalian settings for practical usage. However, the processes affecting gene circuit adaptability have not been systematically investigated. Here we construct a mammalian version of a negative feedback-based ‘linearizer’ gene circuit previously developed in yeast. The first naïve mammalian prototype was non-functional, but a computational model suggested that we could recover function by improving gene expression and protein localization. After rationally developing and combining new parts as the model suggested, we regained function and could tune target gene expression in human cells linearly and precisely as in yeast. The steps we have taken should be generally relevant for transferring any gene circuit from yeast into mammalian cells. Gene circuits created by synthetic biologists working in one system may not be functional when transferred to a different organism. Using computational modelling to identify factors underlying such differences, the authors successfully adapt a yeast ‘linearizer’ circuit so that it functions in mammalian cells." @default.
• W2012175205 created "2016-06-24" @default.
• W2012175205 creator A5050076723 @default.
• W2012175205 creator A5069451179 @default.
• W2012175205 creator A5087385646 @default.
• W2012175205 date "2013-02-05" @default.
• W2012175205 modified "2023-09-30" @default.
• W2012175205 title "Transferring a synthetic gene circuit from yeast to mammalian cells" @default.
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• W2012175205 doi "https://doi.org/10.1038/ncomms2471" @default.
• W2012175205 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3573884" @default.
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