FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012175787> ?p ?o ?g. }

• W2012175787 endingPage "310" @default.
• W2012175787 startingPage "302" @default.
• W2012175787 abstract "An improved surface-immobilisation system was engineered to target heterologous proteins onto vegetative cells and spores of Bacillus thuringiensis plasmid-free recipient strain BMB171. The sporulation-dependent spore cortex-lytic enzyme from B. thuringiensis YBT-1520, SceA, was expressed in vegetative cells and used as the surface anchoring motif. Green fluorescent protein (GFP) and a Bacillus endo-β-1,3-1,4-glucanase (BglS) were used as the fusion partners to test the binding efficiency and the functional activities of immobilised surface proteins. The surface localisation of the SceA-GFP fusion protein on vegetative cells and spores was confirmed by Western blot, immunofluorescence microscopy and flow cytometry. The GFP fluorescence intensity from both vegetative cells and spores was measured and compared to a previously characterised surface display system using a peptidoglycan hydrolase anchor (Mbg). Results demonstrated comparable efficiency of SceA- and Mbg-mediated immobilisation on vegetative cells but a more efficient immobilisation on spores using the SceA anchor, suggesting SceA has greater potential for spore-based applications. The SceA protein was then applied to target BglS onto vegetative cells and spores, and the surface immobilisation was verified by the substantial whole-cell enzymatic activity and enhanced whole-spore enzymatic activity compared to vegetative cells. A dually active B. thuringiensis vegetative cell and spore display system could prove especially valuable for the development of regenerable and heat-stable biocatalysts that function under adverse environmental conditions, for example, an effective feed additive for improved digestion and nutrient absorption by livestock." @default.
• W2012175787 created "2016-06-24" @default.
• W2012175787 creator A5003781835 @default.
• W2012175787 creator A5027155201 @default.
• W2012175787 creator A5056500312 @default.
• W2012175787 creator A5066814137 @default.
• W2012175787 creator A5070719618 @default.
• W2012175787 creator A5078094074 @default.
• W2012175787 creator A5081711721 @default.
• W2012175787 date "2012-02-01" @default.
• W2012175787 modified "2023-10-16" @default.
• W2012175787 title "An improved system for the surface immobilisation of proteins on Bacillus thuringiensis vegetative cells and spores through a new spore cortex-lytic enzyme anchor" @default.
• W2012175787 cites W1494812608 @default.
• W2012175787 cites W1964030197 @default.
• W2012175787 cites W1969448714 @default.
• W2012175787 cites W1975281577 @default.
• W2012175787 cites W1988543890 @default.
• W2012175787 cites W1999734977 @default.
• W2012175787 cites W2001448498 @default.
• W2012175787 cites W2021741281 @default.
• W2012175787 cites W2032222595 @default.
• W2012175787 cites W2036571614 @default.
• W2012175787 cites W2042133316 @default.
• W2012175787 cites W2048079702 @default.
• W2012175787 cites W2054838681 @default.
• W2012175787 cites W2059421675 @default.
• W2012175787 cites W2072959841 @default.
• W2012175787 cites W2074461586 @default.
• W2012175787 cites W2078765633 @default.
• W2012175787 cites W2088248981 @default.
• W2012175787 cites W2096202597 @default.
• W2012175787 cites W2107151561 @default.
• W2012175787 cites W2110144831 @default.
• W2012175787 cites W2124213521 @default.
• W2012175787 cites W2136266813 @default.
• W2012175787 cites W2139050921 @default.
• W2012175787 cites W2142277477 @default.
• W2012175787 cites W2151277682 @default.
• W2012175787 cites W2167443890 @default.
• W2012175787 cites W2168316181 @default.
• W2012175787 cites W2224906727 @default.
• W2012175787 cites W2406218677 @default.
• W2012175787 doi "https://doi.org/10.1016/j.nbt.2011.09.003" @default.
• W2012175787 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21968393" @default.
• W2012175787 hasPublicationYear "2012" @default.
• W2012175787 type Work @default.
• W2012175787 sameAs 2012175787 @default.
• W2012175787 citedByCount "12" @default.
• W2012175787 countsByYear W20121757872013 @default.
• W2012175787 countsByYear W20121757872014 @default.
• W2012175787 countsByYear W20121757872015 @default.
• W2012175787 countsByYear W20121757872016 @default.
• W2012175787 countsByYear W20121757872017 @default.
• W2012175787 countsByYear W20121757872019 @default.
• W2012175787 countsByYear W20121757872020 @default.
• W2012175787 countsByYear W20121757872021 @default.
• W2012175787 crossrefType "journal-article" @default.
• W2012175787 hasAuthorship W2012175787A5003781835 @default.
• W2012175787 hasAuthorship W2012175787A5027155201 @default.
• W2012175787 hasAuthorship W2012175787A5056500312 @default.
• W2012175787 hasAuthorship W2012175787A5066814137 @default.
• W2012175787 hasAuthorship W2012175787A5070719618 @default.
• W2012175787 hasAuthorship W2012175787A5078094074 @default.
• W2012175787 hasAuthorship W2012175787A5081711721 @default.
• W2012175787 hasConcept C104317684 @default.
• W2012175787 hasConcept C123894998 @default.
• W2012175787 hasConcept C142613039 @default.
• W2012175787 hasConcept C174618031 @default.
• W2012175787 hasConcept C181199279 @default.
• W2012175787 hasConcept C185592680 @default.
• W2012175787 hasConcept C2776634448 @default.
• W2012175787 hasConcept C2779035793 @default.
• W2012175787 hasConcept C40767141 @default.
• W2012175787 hasConcept C523546767 @default.
• W2012175787 hasConcept C54355233 @default.
• W2012175787 hasConcept C55493867 @default.
• W2012175787 hasConcept C86803240 @default.
• W2012175787 hasConcept C89423630 @default.
• W2012175787 hasConceptScore W2012175787C104317684 @default.
• W2012175787 hasConceptScore W2012175787C123894998 @default.
• W2012175787 hasConceptScore W2012175787C142613039 @default.
• W2012175787 hasConceptScore W2012175787C174618031 @default.
• W2012175787 hasConceptScore W2012175787C181199279 @default.
• W2012175787 hasConceptScore W2012175787C185592680 @default.
• W2012175787 hasConceptScore W2012175787C2776634448 @default.
• W2012175787 hasConceptScore W2012175787C2779035793 @default.
• W2012175787 hasConceptScore W2012175787C40767141 @default.
• W2012175787 hasConceptScore W2012175787C523546767 @default.
• W2012175787 hasConceptScore W2012175787C54355233 @default.
• W2012175787 hasConceptScore W2012175787C55493867 @default.
• W2012175787 hasConceptScore W2012175787C86803240 @default.
• W2012175787 hasConceptScore W2012175787C89423630 @default.
• W2012175787 hasIssue "3" @default.
• W2012175787 hasLocation W20121757871 @default.
• W2012175787 hasLocation W20121757872 @default.
• W2012175787 hasOpenAccess W2012175787 @default.
• W2012175787 hasPrimaryLocation W20121757871 @default.
• W2012175787 hasRelatedWork W2012175787 @default.

• next