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• W2012176032 abstract "Shiga toxins (STx) are secreted extracellularly through the outer membrane vesicles (OMVs) of Escherichia coli O157:H7. In an attempt to produce STxA-deficient OMVs from E. coli O157:H7, site-specific deletions of the stx1A and stx2A subunit genes were carried out. The STxA-deficient phenotype of the stx1A/stx2A mutant was confirmed by Vero cell cytotoxicity and VTEC-RPLA assay. Western blot analyses showed that the B (STxB) subunits were present without coupling to STxA in the OMVs of the STxA-deficient mutant. Furthermore, STxB was located in its homo-pentameric complexes, as revealed by immunoprecipitation and immunoblotting with anti-STxB antibodies. These results suggest that STxB alone can be oligomerized into the B pentamer in the periplasm, and subsequently entrapped into the OMVs. Determination of the median lethal dose concentration for the OMV preparations suggests that the STxA-deficient OMVs containing STxB complex could be safely used as vaccine delivery vehicles." @default.
• W2012176032 created "2016-06-24" @default.
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• W2012176032 date "2010-04-01" @default.
• W2012176032 modified "2023-09-27" @default.
• W2012176032 title "Shiga toxin A subunit mutant of<i>Escherichia coli</i>O157:H7 releases outer membrane vesicles containing the B-pentameric complex" @default.
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• W2012176032 doi "https://doi.org/10.1111/j.1574-695x.2010.00654.x" @default.
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