FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012194088> ?p ?o ?g. }

• W2012194088 endingPage "211" @default.
• W2012194088 startingPage "192" @default.
• W2012194088 abstract "Microbial functioning refers to microbial activity because only the active microorganisms drive biogeochemical processes. Despite the importance of active microorganisms, most methods focus on estimating total microbial biomass and fail to evaluate its active fraction. At first, we have described the differences among the active, potentially active, and dormant microbial states in soil and suggested threshold values of parameters for their identification. Secondly, we critically reviewed the ability of a broad range of approaches to estimate and characterize the active and the potentially active microorganisms in soil. Following approaches were evaluated: plate count and microbial cultures; direct microscopy combined with cell staining; ATP, PLFA, DNA and RNA content; microarray analyses; PCR-based approaches; stable isotope probing; soil proteomics, enzymes activity; and various approaches based on respiration and substrate utilization. The “static” approaches, mainly based on the single-stage determination of cell components (ATP, DNA, RNA, and molecular biomarkers), detect well the presence of microorganisms and total biomass, but they fail to evaluate the active part and consequently the functions. In contrast, the dynamic approaches, estimating the changes of these parameters during microbial growth and based on process rates: substrate utilization and product formation, e.g., respiration, help to evaluate active microbial biomass and relate it to specific process rates. Based on a comparison of all approaches for their universality (possibility to analyze active, potentially active and dormant microorganisms), we concluded that 1) direct microscopy with complementary stains, 2) a combination of RNA-based FISH with staining of total microbial biomass, and 3) approaches based on microbial growth were the most advantageous and allowed simultaneous quantitative estimation of active, potentially active, and dormant microorganisms in soil. The active microorganisms compose only about 0.1–2% of the total microbial biomass and very seldom exceed 5% in soils without input of easily available substrates. Nonetheless, the fraction of potentially active microorganisms (ready to start utilization of available substrates within few hours) is much higher, contributing between 10 and 40% (up to 60%) of the total microbial biomass. Therefore, we emphasize the role of potentially active microorganisms with quick response to fluctuating substrate input in soil microhabitats and hotspots. The transition from the potentially active to the active state occurs in minutes to hours, but the shift from dormant to active state takes anywhere from hours to days. Despite very fast activation, the reverse process – fading to the potentially active and dormant stage – requires a much longer period and is very different for individual criteria: ATP, DNA, RNA, enzyme production, respiration rates. This leads to further difficulties in the estimation of the active part of microbial community by methods based on these parameters. Consequently, the standardization, further elaboration, and broad application of approaches focused on the portion of active microorganisms in soil and their functions are urgently needed. We conclude that because active microorganisms are the solely microbial drivers of main biogeochemical processes, analyses of the active and potentially active fractions are necessary in studies focused on soil functions." @default.
• W2012194088 created "2016-06-24" @default.
• W2012194088 creator A5062508940 @default.
• W2012194088 creator A5062871768 @default.
• W2012194088 date "2013-12-01" @default.
• W2012194088 modified "2023-10-10" @default.
• W2012194088 title "Active microorganisms in soil: Critical review of estimation criteria and approaches" @default.
• W2012194088 cites W1233072794 @default.
• W2012194088 cites W1513022456 @default.
• W2012194088 cites W1513168266 @default.
• W2012194088 cites W1533811233 @default.
• W2012194088 cites W1548209614 @default.
• W2012194088 cites W1572793332 @default.
• W2012194088 cites W1578440157 @default.
• W2012194088 cites W1760743837 @default.
• W2012194088 cites W1849863192 @default.
• W2012194088 cites W1871667815 @default.
• W2012194088 cites W1873981940 @default.
• W2012194088 cites W1956508283 @default.
• W2012194088 cites W1963583782 @default.
• W2012194088 cites W1965915157 @default.
• W2012194088 cites W1968181582 @default.
• W2012194088 cites W1969193526 @default.
• W2012194088 cites W1971458964 @default.
• W2012194088 cites W1972012129 @default.
• W2012194088 cites W1972852665 @default.
• W2012194088 cites W1973005481 @default.
• W2012194088 cites W1973200192 @default.
• W2012194088 cites W1974836475 @default.
• W2012194088 cites W1974883295 @default.
• W2012194088 cites W1975301557 @default.
• W2012194088 cites W1975472512 @default.
• W2012194088 cites W1976239470 @default.
• W2012194088 cites W1977822184 @default.
• W2012194088 cites W1979015032 @default.
• W2012194088 cites W1980680725 @default.
• W2012194088 cites W1981148285 @default.
• W2012194088 cites W1982475831 @default.
• W2012194088 cites W1982990001 @default.
• W2012194088 cites W1984320817 @default.
• W2012194088 cites W1986834769 @default.
• W2012194088 cites W1988138247 @default.
• W2012194088 cites W1988740945 @default.
• W2012194088 cites W1989789018 @default.
• W2012194088 cites W1990427132 @default.
• W2012194088 cites W1990576133 @default.
• W2012194088 cites W1990779736 @default.
• W2012194088 cites W1991816600 @default.
• W2012194088 cites W1992443677 @default.
• W2012194088 cites W1995253360 @default.
• W2012194088 cites W1998159789 @default.
• W2012194088 cites W1998200993 @default.
• W2012194088 cites W1998220314 @default.
• W2012194088 cites W1998947380 @default.
• W2012194088 cites W2003378402 @default.
• W2012194088 cites W2003738804 @default.
• W2012194088 cites W2005428972 @default.
• W2012194088 cites W2006034635 @default.
• W2012194088 cites W2006387852 @default.
• W2012194088 cites W2007375705 @default.
• W2012194088 cites W2007742841 @default.
• W2012194088 cites W2007994449 @default.
• W2012194088 cites W2008429649 @default.
• W2012194088 cites W2008710085 @default.
• W2012194088 cites W2009421102 @default.
• W2012194088 cites W2011876455 @default.
• W2012194088 cites W2013765373 @default.
• W2012194088 cites W2013820578 @default.
• W2012194088 cites W2017886038 @default.
• W2012194088 cites W2018572001 @default.
• W2012194088 cites W2018841491 @default.
• W2012194088 cites W2020580526 @default.
• W2012194088 cites W2021422645 @default.
• W2012194088 cites W2021513221 @default.
• W2012194088 cites W2023464964 @default.
• W2012194088 cites W2023570154 @default.
• W2012194088 cites W2023831837 @default.
• W2012194088 cites W2025370873 @default.
• W2012194088 cites W2027074178 @default.
• W2012194088 cites W2027438294 @default.
• W2012194088 cites W2027674313 @default.
• W2012194088 cites W2029154455 @default.
• W2012194088 cites W2030659432 @default.
• W2012194088 cites W2034748833 @default.
• W2012194088 cites W2034836989 @default.
• W2012194088 cites W2036120465 @default.
• W2012194088 cites W2037273958 @default.
• W2012194088 cites W2037952987 @default.
• W2012194088 cites W2039919426 @default.
• W2012194088 cites W2041044721 @default.
• W2012194088 cites W2041128052 @default.
• W2012194088 cites W2041352649 @default.
• W2012194088 cites W2041577010 @default.
• W2012194088 cites W2043461906 @default.
• W2012194088 cites W2046642933 @default.
• W2012194088 cites W2046965598 @default.
• W2012194088 cites W2048350517 @default.
• W2012194088 cites W2048732522 @default.

• next