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- W2012194131 abstract "The present study characterizes the effects of the boric acid binding on calf thymus DNA (ct-DNA) by spectroscopic and calorimetric methods. UV–Vis absorbance spectroscopy, circular dichroism (CD) spectroscopy, transmission electron microscopy (TEM), isothermal titration calorimetry (ITC), and Fourier transform infrared (FT-IR) spectroscopy were employed to characterize binding properties. Changes in the secondary structure of ct-DNA were determined by CD spectroscopy. Sizes and morphologies of boric acid–DNA complexes were determined by transmission electron microscopy (TEM). The kinetics of boric acid binding to calf thymus DNA (ct-DNA) was investigated by isothermal titration calorimetry (ITC). ITC results revealed that boric acid exhibits a moderate affinity to ct-DNA with a binding constant (K a) of 9.54 × 104 M−1. FT-IR results revealed that boric acid binds to the deoxyribose sugar of DNA without disrupting the B-conformation at tested concentrations." @default.
- W2012194131 created "2016-06-24" @default.
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- W2012194131 date "2014-03-21" @default.
- W2012194131 modified "2023-09-25" @default.
- W2012194131 title "Non-intercalative, Deoxyribose Binding of Boric Acid to Calf Thymus DNA" @default.
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- W2012194131 doi "https://doi.org/10.1007/s12011-014-9924-8" @default.
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