Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012195188> ?p ?o ?g. }
- W2012195188 endingPage "269" @default.
- W2012195188 startingPage "263" @default.
- W2012195188 abstract "3-Deoxy-d-arabino-heptulosonate-7-phosphate synthase (DAHPS), (EC 2.5.1.54) catalyzes the first step of the shikimate pathway, the route for the biosynthesis of aromatic compounds in plants and microbes. In Actinosynnema pretiosum, the aroF gene (GenBank: AF056968.1) encodes DAHPS to condensate phosphoenolpyruvate (PEP) and d-erythrose 4-phosphate (E4P) to generate DAHP. In this study, a recombinant pET28a-aroF plasmid was constructed and A. pretiosum DAHPS was successfully expressed in soluble form by co-expression with chaperonins GroEL/GroES in Escherichia coli. The purification and kinetic characterization of the expressed protein were then investigated. The DAHPS originated from A. pretiosum demonstrated a pronounced substrate inhibition by PEP but was not sensitive to E4P. The purified enzyme was completely inactivated by EDTA but potently activated by several bivalent metal ions, especially Mn(2+) and Co(2+)." @default.
- W2012195188 created "2016-06-24" @default.
- W2012195188 creator A5025046240 @default.
- W2012195188 creator A5035586526 @default.
- W2012195188 creator A5042699380 @default.
- W2012195188 creator A5045803827 @default.
- W2012195188 date "2012-04-01" @default.
- W2012195188 modified "2023-10-18" @default.
- W2012195188 title "Heterologous expression and characterization of soluble recombinant 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase from Actinosynnema pretiosum ssp. auranticum ATCC31565 through co-expression with Chaperones in Escherichia coli" @default.
- W2012195188 cites W1530364531 @default.
- W2012195188 cites W1559420025 @default.
- W2012195188 cites W1620758289 @default.
- W2012195188 cites W1660123129 @default.
- W2012195188 cites W1884643618 @default.
- W2012195188 cites W1935952679 @default.
- W2012195188 cites W1965764842 @default.
- W2012195188 cites W1966950377 @default.
- W2012195188 cites W1973244479 @default.
- W2012195188 cites W1973769186 @default.
- W2012195188 cites W1985257351 @default.
- W2012195188 cites W2011019587 @default.
- W2012195188 cites W2017818060 @default.
- W2012195188 cites W2023109286 @default.
- W2012195188 cites W2027294575 @default.
- W2012195188 cites W2055485700 @default.
- W2012195188 cites W2073065868 @default.
- W2012195188 cites W2078952292 @default.
- W2012195188 cites W2091262881 @default.
- W2012195188 cites W2094123239 @default.
- W2012195188 cites W2100837269 @default.
- W2012195188 cites W2106857639 @default.
- W2012195188 cites W2122858000 @default.
- W2012195188 cites W2133731751 @default.
- W2012195188 cites W2141426247 @default.
- W2012195188 cites W2141487322 @default.
- W2012195188 cites W2158113700 @default.
- W2012195188 cites W4293247451 @default.
- W2012195188 doi "https://doi.org/10.1016/j.pep.2012.01.013" @default.
- W2012195188 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22326798" @default.
- W2012195188 hasPublicationYear "2012" @default.
- W2012195188 type Work @default.
- W2012195188 sameAs 2012195188 @default.
- W2012195188 citedByCount "9" @default.
- W2012195188 countsByYear W20121951882012 @default.
- W2012195188 countsByYear W20121951882013 @default.
- W2012195188 countsByYear W20121951882014 @default.
- W2012195188 countsByYear W20121951882015 @default.
- W2012195188 countsByYear W20121951882016 @default.
- W2012195188 countsByYear W20121951882019 @default.
- W2012195188 countsByYear W20121951882020 @default.
- W2012195188 crossrefType "journal-article" @default.
- W2012195188 hasAuthorship W2012195188A5025046240 @default.
- W2012195188 hasAuthorship W2012195188A5035586526 @default.
- W2012195188 hasAuthorship W2012195188A5042699380 @default.
- W2012195188 hasAuthorship W2012195188A5045803827 @default.
- W2012195188 hasConcept C104317684 @default.
- W2012195188 hasConcept C108305142 @default.
- W2012195188 hasConcept C112243037 @default.
- W2012195188 hasConcept C143937172 @default.
- W2012195188 hasConcept C153911025 @default.
- W2012195188 hasConcept C181199279 @default.
- W2012195188 hasConcept C185592680 @default.
- W2012195188 hasConcept C2778255928 @default.
- W2012195188 hasConcept C33161422 @default.
- W2012195188 hasConcept C40767141 @default.
- W2012195188 hasConcept C46522908 @default.
- W2012195188 hasConcept C547475151 @default.
- W2012195188 hasConcept C55493867 @default.
- W2012195188 hasConcept C85755625 @default.
- W2012195188 hasConcept C86803240 @default.
- W2012195188 hasConcept C87190427 @default.
- W2012195188 hasConceptScore W2012195188C104317684 @default.
- W2012195188 hasConceptScore W2012195188C108305142 @default.
- W2012195188 hasConceptScore W2012195188C112243037 @default.
- W2012195188 hasConceptScore W2012195188C143937172 @default.
- W2012195188 hasConceptScore W2012195188C153911025 @default.
- W2012195188 hasConceptScore W2012195188C181199279 @default.
- W2012195188 hasConceptScore W2012195188C185592680 @default.
- W2012195188 hasConceptScore W2012195188C2778255928 @default.
- W2012195188 hasConceptScore W2012195188C33161422 @default.
- W2012195188 hasConceptScore W2012195188C40767141 @default.
- W2012195188 hasConceptScore W2012195188C46522908 @default.
- W2012195188 hasConceptScore W2012195188C547475151 @default.
- W2012195188 hasConceptScore W2012195188C55493867 @default.
- W2012195188 hasConceptScore W2012195188C85755625 @default.
- W2012195188 hasConceptScore W2012195188C86803240 @default.
- W2012195188 hasConceptScore W2012195188C87190427 @default.
- W2012195188 hasFunder F4320309612 @default.
- W2012195188 hasFunder F4320335766 @default.
- W2012195188 hasFunder F4320335777 @default.
- W2012195188 hasIssue "2" @default.
- W2012195188 hasLocation W20121951881 @default.
- W2012195188 hasLocation W20121951882 @default.
- W2012195188 hasOpenAccess W2012195188 @default.
- W2012195188 hasPrimaryLocation W20121951881 @default.
- W2012195188 hasRelatedWork W1566678551 @default.
- W2012195188 hasRelatedWork W1964572802 @default.
- W2012195188 hasRelatedWork W2011798619 @default.