FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012197995> ?p ?o ?g. }

• W2012197995 endingPage "2026" @default.
• W2012197995 startingPage "2013" @default.
• W2012197995 abstract "Noscapine is a phthalideisoquinoline alkaloid investigated for its potent pharmacological properties. Although structurally elucidated more than a century ago, the biosynthesis of noscapine has not been established. Radiotracer studies have shown that noscapine is derived from the protoberberine alkaloid (S)-scoulerine and has been proposed to proceed through (S)-N-methylcanadine. However, pathway intermediates involved in the conversion of N-methylcanadine to noscapine have not been identified. We report the isolation and characterization of the cytochrome P-450 CYP82Y1, which catalyzes the 1-hydroxylation of N-methylcanadine to 1-hydroxy-N-methylcanadine. Comparison of transcript and metabolite profiles of eight opium poppy chemotypes revealed four cytochrome P-450s, three from the CYP82 and one from the CYP719 families, that were tightly correlated with noscapine accumulation. Recombinant CYP82Y1 was the only enzyme that accepted (R,S)-N-methylcanadine as a substrate with strict specificity and high affinity. As expected, CYP82Y1 was abundantly expressed in opium poppy stems where noscapine accumulation is highest among plant organs. Suppression of CYP82Y1 using virus-induced gene silencing caused a significant reduction in the levels of noscapine, narcotoline, and a putative downstream secoberbine intermediate and also resulted in increased accumulation of the upstream pathway intermediates scoulerine, tetrahydrocolum-bamine, canadine, and N-methylcanadine. The combined biochemical and physiological data support the 1-hydroxylation of (S)-N-methylcanadine catalyzed by CYP82Y1 as the first committed step in the formation of noscapine in opium poppy." @default.
• W2012197995 created "2016-06-24" @default.
• W2012197995 creator A5020766540 @default.
• W2012197995 creator A5056457115 @default.
• W2012197995 date "2014-01-01" @default.
• W2012197995 modified "2023-10-14" @default.
• W2012197995 title "CYP82Y1 Is N-Methylcanadine 1-Hydroxylase, a Key Noscapine Biosynthetic Enzyme in Opium Poppy" @default.
• W2012197995 cites W1480262691 @default.
• W2012197995 cites W1515404266 @default.
• W2012197995 cites W1662769244 @default.
• W2012197995 cites W1964760806 @default.
• W2012197995 cites W1970785350 @default.
• W2012197995 cites W1972727051 @default.
• W2012197995 cites W1975484145 @default.
• W2012197995 cites W1980698982 @default.
• W2012197995 cites W1983444305 @default.
• W2012197995 cites W1985907407 @default.
• W2012197995 cites W1986544564 @default.
• W2012197995 cites W1989812904 @default.
• W2012197995 cites W2008726372 @default.
• W2012197995 cites W2016373857 @default.
• W2012197995 cites W2018575459 @default.
• W2012197995 cites W2021594707 @default.
• W2012197995 cites W2034563867 @default.
• W2012197995 cites W2038177381 @default.
• W2012197995 cites W2038735800 @default.
• W2012197995 cites W2066963004 @default.
• W2012197995 cites W2067279194 @default.
• W2012197995 cites W2078768180 @default.
• W2012197995 cites W2079038249 @default.
• W2012197995 cites W2079053455 @default.
• W2012197995 cites W2091493196 @default.
• W2012197995 cites W2094528776 @default.
• W2012197995 cites W2096851866 @default.
• W2012197995 cites W2100216377 @default.
• W2012197995 cites W2112762465 @default.
• W2012197995 cites W2124847289 @default.
• W2012197995 cites W2127800696 @default.
• W2012197995 cites W2137015675 @default.
• W2012197995 cites W2140613686 @default.
• W2012197995 cites W2149383303 @default.
• W2012197995 cites W2158948449 @default.
• W2012197995 cites W2334063808 @default.
• W2012197995 doi "https://doi.org/10.1074/jbc.m113.505099" @default.
• W2012197995 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3900951" @default.
• W2012197995 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24324259" @default.
• W2012197995 hasPublicationYear "2014" @default.
• W2012197995 type Work @default.
• W2012197995 sameAs 2012197995 @default.
• W2012197995 citedByCount "43" @default.
• W2012197995 countsByYear W20121979952014 @default.
• W2012197995 countsByYear W20121979952015 @default.
• W2012197995 countsByYear W20121979952016 @default.
• W2012197995 countsByYear W20121979952017 @default.
• W2012197995 countsByYear W20121979952018 @default.
• W2012197995 countsByYear W20121979952019 @default.
• W2012197995 countsByYear W20121979952020 @default.
• W2012197995 countsByYear W20121979952021 @default.
• W2012197995 countsByYear W20121979952022 @default.
• W2012197995 countsByYear W20121979952023 @default.
• W2012197995 crossrefType "journal-article" @default.
• W2012197995 hasAuthorship W2012197995A5020766540 @default.
• W2012197995 hasAuthorship W2012197995A5056457115 @default.
• W2012197995 hasBestOaLocation W20121979951 @default.
• W2012197995 hasConcept C181199279 @default.
• W2012197995 hasConcept C185592680 @default.
• W2012197995 hasConcept C2776295790 @default.
• W2012197995 hasConcept C2777423364 @default.
• W2012197995 hasConcept C2777477808 @default.
• W2012197995 hasConcept C2777667214 @default.
• W2012197995 hasConcept C2778688723 @default.
• W2012197995 hasConcept C2779200813 @default.
• W2012197995 hasConcept C2779733990 @default.
• W2012197995 hasConcept C2781109383 @default.
• W2012197995 hasConcept C526171541 @default.
• W2012197995 hasConcept C553450214 @default.
• W2012197995 hasConcept C55493867 @default.
• W2012197995 hasConcept C59822182 @default.
• W2012197995 hasConcept C71240020 @default.
• W2012197995 hasConcept C86803240 @default.
• W2012197995 hasConceptScore W2012197995C181199279 @default.
• W2012197995 hasConceptScore W2012197995C185592680 @default.
• W2012197995 hasConceptScore W2012197995C2776295790 @default.
• W2012197995 hasConceptScore W2012197995C2777423364 @default.
• W2012197995 hasConceptScore W2012197995C2777477808 @default.
• W2012197995 hasConceptScore W2012197995C2777667214 @default.
• W2012197995 hasConceptScore W2012197995C2778688723 @default.
• W2012197995 hasConceptScore W2012197995C2779200813 @default.
• W2012197995 hasConceptScore W2012197995C2779733990 @default.
• W2012197995 hasConceptScore W2012197995C2781109383 @default.
• W2012197995 hasConceptScore W2012197995C526171541 @default.
• W2012197995 hasConceptScore W2012197995C553450214 @default.
• W2012197995 hasConceptScore W2012197995C55493867 @default.
• W2012197995 hasConceptScore W2012197995C59822182 @default.
• W2012197995 hasConceptScore W2012197995C71240020 @default.
• W2012197995 hasConceptScore W2012197995C86803240 @default.
• W2012197995 hasIssue "4" @default.
• W2012197995 hasLocation W20121979951 @default.

• next