FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012199851> ?p ?o ?g. }

• W2012199851 endingPage "145" @default.
• W2012199851 startingPage "140" @default.
• W2012199851 abstract "A series of experiments was performed to compare the ability of adult human endothelial and mesothelial cells to attach and spread upon ePTFE graft material. Both cell types were harvested enzymatically and grown in culture. Two types of adhesion assay were used to assess the ability of the cultured cells to attach to ePTFE either uncoated or precoated with a variety of substrate proteins. The results showed that fibronectin, laminin, type 4 collagen and preclotted blood all greatly improve the attachment rate of endothelium to ePTFE. The extracellular matrix proteins, however, give patchy cell attachment, whereas cells seeded onto preclotted ePTFE form a virtually confluent monolayer after one hour's incubation. The matrix proteins similarly improve mesothelial attachment, although in all cases attached cells remain largely rounded up, with relatively few spreading on the surface. Mesothelial attachment to preclotted ePTFE is no better than attachment to the untreated material. It would seem that a preclotted graft seeded with endothelial cells is the combination most likely to result in a confluent monolayer within one hour. A series of experiments was performed to compare the ability of adult human endothelial and mesothelial cells to attach and spread upon ePTFE graft material. Both cell types were harvested enzymatically and grown in culture. Two types of adhesion assay were used to assess the ability of the cultured cells to attach to ePTFE either uncoated or precoated with a variety of substrate proteins. The results showed that fibronectin, laminin, type 4 collagen and preclotted blood all greatly improve the attachment rate of endothelium to ePTFE. The extracellular matrix proteins, however, give patchy cell attachment, whereas cells seeded onto preclotted ePTFE form a virtually confluent monolayer after one hour's incubation. The matrix proteins similarly improve mesothelial attachment, although in all cases attached cells remain largely rounded up, with relatively few spreading on the surface. Mesothelial attachment to preclotted ePTFE is no better than attachment to the untreated material. It would seem that a preclotted graft seeded with endothelial cells is the combination most likely to result in a confluent monolayer within one hour." @default.
• W2012199851 created "2016-06-24" @default.
• W2012199851 creator A5001854589 @default.
• W2012199851 creator A5058001525 @default.
• W2012199851 creator A5067407609 @default.
• W2012199851 date "1989-04-01" @default.
• W2012199851 modified "2023-10-03" @default.
• W2012199851 title "Cell Seeding for Small Diameter ePTFE Vascular Grafts: A Comparison Between Adult Human Endothelial and Mesothelial Cells" @default.
• W2012199851 cites W2025208026 @default.
• W2012199851 cites W2089907307 @default.
• W2012199851 cites W2117972619 @default.
• W2012199851 cites W2470982991 @default.
• W2012199851 doi "https://doi.org/10.1016/s0890-5096(06)62007-4" @default.
• W2012199851 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2765355" @default.
• W2012199851 hasPublicationYear "1989" @default.
• W2012199851 type Work @default.
• W2012199851 sameAs 2012199851 @default.
• W2012199851 citedByCount "24" @default.
• W2012199851 countsByYear W20121998512013 @default.
• W2012199851 countsByYear W20121998512016 @default.
• W2012199851 countsByYear W20121998512020 @default.
• W2012199851 countsByYear W20121998512022 @default.
• W2012199851 crossrefType "journal-article" @default.
• W2012199851 hasAuthorship W2012199851A5001854589 @default.
• W2012199851 hasAuthorship W2012199851A5058001525 @default.
• W2012199851 hasAuthorship W2012199851A5067407609 @default.
• W2012199851 hasConcept C106487976 @default.
• W2012199851 hasConcept C123012128 @default.
• W2012199851 hasConcept C136229726 @default.
• W2012199851 hasConcept C142724271 @default.
• W2012199851 hasConcept C178790620 @default.
• W2012199851 hasConcept C185592680 @default.
• W2012199851 hasConcept C189165786 @default.
• W2012199851 hasConcept C202751555 @default.
• W2012199851 hasConcept C2779747549 @default.
• W2012199851 hasConcept C2779814568 @default.
• W2012199851 hasConcept C2909640154 @default.
• W2012199851 hasConcept C43617362 @default.
• W2012199851 hasConcept C55493867 @default.
• W2012199851 hasConcept C71924100 @default.
• W2012199851 hasConcept C84416704 @default.
• W2012199851 hasConcept C85789140 @default.
• W2012199851 hasConcept C86492073 @default.
• W2012199851 hasConcept C86803240 @default.
• W2012199851 hasConcept C95444343 @default.
• W2012199851 hasConceptScore W2012199851C106487976 @default.
• W2012199851 hasConceptScore W2012199851C123012128 @default.
• W2012199851 hasConceptScore W2012199851C136229726 @default.
• W2012199851 hasConceptScore W2012199851C142724271 @default.
• W2012199851 hasConceptScore W2012199851C178790620 @default.
• W2012199851 hasConceptScore W2012199851C185592680 @default.
• W2012199851 hasConceptScore W2012199851C189165786 @default.
• W2012199851 hasConceptScore W2012199851C202751555 @default.
• W2012199851 hasConceptScore W2012199851C2779747549 @default.
• W2012199851 hasConceptScore W2012199851C2779814568 @default.
• W2012199851 hasConceptScore W2012199851C2909640154 @default.
• W2012199851 hasConceptScore W2012199851C43617362 @default.
• W2012199851 hasConceptScore W2012199851C55493867 @default.
• W2012199851 hasConceptScore W2012199851C71924100 @default.
• W2012199851 hasConceptScore W2012199851C84416704 @default.
• W2012199851 hasConceptScore W2012199851C85789140 @default.
• W2012199851 hasConceptScore W2012199851C86492073 @default.
• W2012199851 hasConceptScore W2012199851C86803240 @default.
• W2012199851 hasConceptScore W2012199851C95444343 @default.
• W2012199851 hasIssue "2" @default.
• W2012199851 hasLocation W20121998511 @default.
• W2012199851 hasLocation W20121998512 @default.
• W2012199851 hasOpenAccess W2012199851 @default.
• W2012199851 hasPrimaryLocation W20121998511 @default.
• W2012199851 hasRelatedWork W2007650541 @default.
• W2012199851 hasRelatedWork W2013466449 @default.
• W2012199851 hasRelatedWork W2021751632 @default.
• W2012199851 hasRelatedWork W2041906104 @default.
• W2012199851 hasRelatedWork W2080235403 @default.
• W2012199851 hasRelatedWork W2085862310 @default.
• W2012199851 hasRelatedWork W2106761248 @default.
• W2012199851 hasRelatedWork W2128518557 @default.
• W2012199851 hasRelatedWork W2545086193 @default.
• W2012199851 hasRelatedWork W4256388796 @default.
• W2012199851 hasVolume "3" @default.
• W2012199851 isParatext "false" @default.
• W2012199851 isRetracted "false" @default.
• W2012199851 magId "2012199851" @default.
• W2012199851 workType "article" @default.