Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012201168> ?p ?o ?g. }
- W2012201168 endingPage "261" @default.
- W2012201168 startingPage "251" @default.
- W2012201168 abstract "Rbfox RNA binding proteins are implicated as regulators of phylogenetically-conserved alternative splicing events important for muscle function. To investigate the function of rbfox genes, we used morpholino-mediated knockdown of muscle-expressed rbfox1l and rbfox2 in zebrafish embryos. Single and double morphant embryos exhibited changes in splicing of overlapping sets of bioinformatically-predicted rbfox target exons, many of which exhibit a muscle-enriched splicing pattern that is conserved in vertebrates. Thus, conservation of intronic Rbfox binding motifs is a good predictor of Rbfox-regulated alternative splicing. Morphology and development of single morphant embryos were strikingly normal; however, muscle development in double morphants was severely disrupted. Defects in cardiac muscle were marked by reduced heart rate and in skeletal muscle by complete paralysis. The predominance of wavy myofibers and abnormal thick and thin filaments in skeletal muscle revealed that myofibril assembly is defective and disorganized in double morphants. Ultra-structural analysis revealed that although sarcomeres with electron dense M- and Z-bands are present in muscle fibers of rbfox1l/rbox2 morphants, they are substantially reduced in number and alignment. Importantly, splicing changes and morphological defects were rescued by expression of morpholino-resistant rbfox cDNA. Additionally, a target-blocking MO complementary to a single UGCAUG motif adjacent to an rbfox target exon of fxr1 inhibited inclusion in a similar manner to rbfox knockdown, providing evidence that Rbfox regulates the splicing of target exons via direct binding to intronic regulatory motifs. We conclude that Rbfox proteins regulate an alternative splicing program essential for vertebrate heart and skeletal muscle functions." @default.
- W2012201168 created "2016-06-24" @default.
- W2012201168 creator A5008632337 @default.
- W2012201168 creator A5023472472 @default.
- W2012201168 creator A5029850300 @default.
- W2012201168 creator A5036061323 @default.
- W2012201168 creator A5037151010 @default.
- W2012201168 creator A5038048002 @default.
- W2012201168 creator A5057220704 @default.
- W2012201168 creator A5057675017 @default.
- W2012201168 creator A5065360516 @default.
- W2012201168 creator A5068352204 @default.
- W2012201168 creator A5091640988 @default.
- W2012201168 date "2011-11-01" @default.
- W2012201168 modified "2023-09-30" @default.
- W2012201168 title "Rbfox-regulated alternative splicing is critical for zebrafish cardiac and skeletal muscle functions" @default.
- W2012201168 cites W1947859348 @default.
- W2012201168 cites W1964005742 @default.
- W2012201168 cites W1964496833 @default.
- W2012201168 cites W1966663468 @default.
- W2012201168 cites W1967711973 @default.
- W2012201168 cites W1969707555 @default.
- W2012201168 cites W1974951335 @default.
- W2012201168 cites W197715220 @default.
- W2012201168 cites W1979718394 @default.
- W2012201168 cites W1983847126 @default.
- W2012201168 cites W1985206041 @default.
- W2012201168 cites W1994025422 @default.
- W2012201168 cites W1997668305 @default.
- W2012201168 cites W2004363672 @default.
- W2012201168 cites W2008163521 @default.
- W2012201168 cites W2011920499 @default.
- W2012201168 cites W2016278733 @default.
- W2012201168 cites W2024562641 @default.
- W2012201168 cites W2031048947 @default.
- W2012201168 cites W2038617558 @default.
- W2012201168 cites W2041168550 @default.
- W2012201168 cites W2041604586 @default.
- W2012201168 cites W2044146407 @default.
- W2012201168 cites W2049904722 @default.
- W2012201168 cites W2052038494 @default.
- W2012201168 cites W2063530272 @default.
- W2012201168 cites W2069543630 @default.
- W2012201168 cites W2069658044 @default.
- W2012201168 cites W2079643255 @default.
- W2012201168 cites W2082472219 @default.
- W2012201168 cites W2093169204 @default.
- W2012201168 cites W2093506762 @default.
- W2012201168 cites W2093724290 @default.
- W2012201168 cites W2093881462 @default.
- W2012201168 cites W2096251733 @default.
- W2012201168 cites W2102887078 @default.
- W2012201168 cites W2104657885 @default.
- W2012201168 cites W2106902692 @default.
- W2012201168 cites W2107385913 @default.
- W2012201168 cites W2109686700 @default.
- W2012201168 cites W2110459103 @default.
- W2012201168 cites W2113525941 @default.
- W2012201168 cites W2118064602 @default.
- W2012201168 cites W2118305000 @default.
- W2012201168 cites W2120216148 @default.
- W2012201168 cites W2121816001 @default.
- W2012201168 cites W2121908416 @default.
- W2012201168 cites W2124169580 @default.
- W2012201168 cites W2124182320 @default.
- W2012201168 cites W2125295969 @default.
- W2012201168 cites W2125349610 @default.
- W2012201168 cites W2131587855 @default.
- W2012201168 cites W2138846202 @default.
- W2012201168 cites W2149116589 @default.
- W2012201168 cites W2149174656 @default.
- W2012201168 cites W2151256435 @default.
- W2012201168 cites W2155804510 @default.
- W2012201168 cites W2158982822 @default.
- W2012201168 cites W2164307088 @default.
- W2012201168 cites W2168110204 @default.
- W2012201168 cites W2170648070 @default.
- W2012201168 cites W2171402579 @default.
- W2012201168 doi "https://doi.org/10.1016/j.ydbio.2011.08.025" @default.
- W2012201168 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3521583" @default.
- W2012201168 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21925157" @default.
- W2012201168 hasPublicationYear "2011" @default.
- W2012201168 type Work @default.
- W2012201168 sameAs 2012201168 @default.
- W2012201168 citedByCount "82" @default.
- W2012201168 countsByYear W20122011682013 @default.
- W2012201168 countsByYear W20122011682014 @default.
- W2012201168 countsByYear W20122011682015 @default.
- W2012201168 countsByYear W20122011682016 @default.
- W2012201168 countsByYear W20122011682017 @default.
- W2012201168 countsByYear W20122011682018 @default.
- W2012201168 countsByYear W20122011682019 @default.
- W2012201168 countsByYear W20122011682020 @default.
- W2012201168 countsByYear W20122011682021 @default.
- W2012201168 countsByYear W20122011682022 @default.
- W2012201168 countsByYear W20122011682023 @default.
- W2012201168 crossrefType "journal-article" @default.
- W2012201168 hasAuthorship W2012201168A5008632337 @default.