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• W2012207172 startingPage "e7560" @default.
• W2012207172 abstract "Cell nuclei are commonly isolated and studied in media which include millimolar concentrations of cations, which conserve the nuclear volume by screening the negative charges on chromatin and maintaining its compaction. However, two factors question if these ionic conditions correctly reproduce the environment of nuclei in vivo: the small-scale motion and conformation of chromatin in vivo are not reproduced in isolated nuclei, and experiments and theory suggest that small ions in the cytoplasm are not free in the soluble phase but are predominantly bound to macromolecules. We studied the possible role in maintaining the structure and functions of nuclei in vivo of a further but frequently overlooked property of the cytoplasm, the crowding or osmotic effects caused by diffusible macromolecules whose concentration, measured in several studies, is in the range of 130 mg/ml. Nuclei which conserved their volume in the cell and their ultrastructure seen by electron microscopy were released from K562 cells in media containing the inert polymer 70 kDa Ficoll (50% w/v) or 70 kDa dextran (35% w/v) to replace the diffusible cytoplasmic molecules which were dispersed on cell lysis with digitonin, with 100 microM K-Hepes buffer as the only source of ions. Immunofluorescence labelling and experiments using cells expressing GFP-fusion proteins showed that internal compartments (nucleoli, PML and coiled bodies, foci of RNA polymerase II) were conserved in these nuclei, and nascent RNA transcripts could be elongated. Our observations are consistent with the hypothesis that crowding by diffusible cytoplasmic macromolecules is a crucial but overlooked factor which supports the nucleus in vivo by equilibrating the opposing osmotic pressure cause by the high concentration of macromolecules in the nucleus, and suggest that crowded media provide more physiological conditions to study nuclear structure and functions. They may also help to resolve the long-standing paradox that the small-scale motion and irregular conformation of chromatin seen in vivo are not reproduced in nuclei isolated in conventional ionic media." @default.
• W2012207172 created "2016-06-24" @default.
• W2012207172 creator A5048268944 @default.
• W2012207172 creator A5048541830 @default.
• W2012207172 date "2009-10-23" @default.
• W2012207172 modified "2023-09-27" @default.
• W2012207172 title "Isolation of Cell Nuclei Using Inert Macromolecules to Mimic the Crowded Cytoplasm" @default.
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• W2012207172 cites W1874943101 @default.
• W2012207172 cites W1909883720 @default.
• W2012207172 cites W1968894421 @default.
• W2012207172 cites W1971586666 @default.
• W2012207172 cites W1972263201 @default.
• W2012207172 cites W1972732859 @default.
• W2012207172 cites W1975747160 @default.
• W2012207172 cites W1979799065 @default.
• W2012207172 cites W1981878314 @default.
• W2012207172 cites W1982020650 @default.
• W2012207172 cites W1983369082 @default.
• W2012207172 cites W1986766805 @default.
• W2012207172 cites W1988307037 @default.
• W2012207172 cites W1991680099 @default.
• W2012207172 cites W1993659912 @default.
• W2012207172 cites W2002044115 @default.
• W2012207172 cites W2004824571 @default.
• W2012207172 cites W2008015588 @default.
• W2012207172 cites W2021883083 @default.
• W2012207172 cites W2025059119 @default.
• W2012207172 cites W2025974342 @default.
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• W2012207172 cites W2034990824 @default.
• W2012207172 cites W2044647218 @default.
• W2012207172 cites W2048920136 @default.
• W2012207172 cites W2056293409 @default.
• W2012207172 cites W2060512844 @default.
• W2012207172 cites W2061108475 @default.
• W2012207172 cites W2066438071 @default.
• W2012207172 cites W2066495646 @default.
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• W2012207172 cites W2077398788 @default.
• W2012207172 cites W2079820907 @default.
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• W2012207172 cites W2088759580 @default.
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• W2012207172 cites W2090783666 @default.
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• W2012207172 doi "https://doi.org/10.1371/journal.pone.0007560" @default.
• W2012207172 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2762040" @default.
• W2012207172 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19851505" @default.
• W2012207172 hasPublicationYear "2009" @default.
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