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- W2012241569 abstract "A simple and efficient method utilizing in vivo recombination to create recombinant libraries incorporating the products of PCR amplification is described. This will be especially useful for generating large pools of randomly mutagenized clones after error-prone PCR mutagenesis. Here we investigate various parameters to optimize this approach and we demonstrate that as little as1 pmole of PCR fragment can generate a library with greater than 104 clones in a single transformation without ligation." @default.
- W2012241569 created "2016-06-24" @default.
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- W2012241569 date "2010-09-01" @default.
- W2012241569 modified "2023-10-09" @default.
- W2012241569 title "Rapid generation of random mutant libraries" @default.
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- W2012241569 doi "https://doi.org/10.4161/bbug.1.5.12942" @default.
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