FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012244911> ?p ?o ?g. }

• W2012244911 endingPage "2783" @default.
• W2012244911 startingPage "2783" @default.
• W2012244911 abstract "Acceleration of gene transfection efficiency in neuroblastoma cells through polyethyleneimine/poly(methyl methacrylate) core-shell magnetic nanoparticles Tewin Tencomnao,1,* Kewalin Klangthong,2,* Nuttaporn Pimpha,3 Saowaluk Chaleawlert-umpon,3 Somsak Saesoo,3 Noppawan Woramongkolchai,3 Nattika Saengkrit,31Center for Excellence in Omics-Nano Medical Technology Development Project, 2Graduate Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 3National Nanotechnology Center, National Science and Technology Development Agency, Pathumthani, Thailand*Both authors contributed equally to this workBackground: The purpose of this study was to demonstrate the potential of magnetic poly(methyl methacrylate) (PMMA) core/polyethyleneimine (PEI) shell (mag-PEI) nanoparticles, which possess high saturation magnetization for gene delivery. By using mag-PEI nanoparticles as a gene carrier, this study focused on evaluation of transfection efficiency under magnetic induction. The potential role of this newly synthesized nanosphere for therapeutic delivery of the tryptophan hydroxylase-2 (TPH-2) gene was also investigated in cultured neuronal LAN-5 cells.Methods: The mag-PEI nanoparticles were prepared by one-step emulsifier-free emulsion polymerization, generating highly loaded and monodispersed magnetic polymeric nanoparticles bearing an amine group. The physicochemical properties of the mag-PEI nanoparticles and DNA-bound mag-PEI nanoparticles were investigated using the gel retardation assay, atomic force microscopy, and zeta size measurements. The gene transfection efficiencies of mag-PEI nanoparticles were evaluated at different transfection times. Confocal laser scanning microscopy confirmed intracellular uptake of the magnetoplex. The optimal conditions for transfection of TPH-2 were selected for therapeutic gene transfection. We isolated the TPH-2 gene from the total RNA of the human medulla oblongata and cloned it into an expression vector. The plasmid containing TPH-2 was subsequently bound onto the surfaces of the mag-PEI nanoparticles via electrostatic interaction. Finally, the mag-PEI nanoparticle magnetoplex was delivered into LAN-5 cells. Reverse-transcriptase polymerase chain reaction was performed to evaluate TPH-2 expression in a quantitative manner.Results: The study demonstrated the role of newly synthesized high-magnetization mag-PEI nanoparticles for gene transfection in vitro. The expression signals of a model gene, luciferase, and a therapeutic gene, TPH-2, were enhanced under magnetic-assisted transfection. An in vitro study in neuronal cells confirmed that using mag-PEI nanoparticles as a DNA carrier for gene delivery provided high transfection efficiency with low cytotoxicity.Conclusion: The mag-PEI nanoparticle is a promising alternative gene transfection reagent due to its ease of use, effectiveness, and low cellular toxicity. The mag-PEI nanoparticle is not only practical for gene transfection in cultured neuronal cells but may also be suitable for transfection in other cells as well.Keywords: magnetic nanoparticle, non-viral vector, gene delivery, tryptophan hydroxylase-2, LAN-5, neuronal cells" @default.
• W2012244911 created "2016-06-24" @default.
• W2012244911 creator A5024051438 @default.
• W2012244911 creator A5039159399 @default.
• W2012244911 creator A5049224975 @default.
• W2012244911 creator A5051111931 @default.
• W2012244911 creator A5056916296 @default.
• W2012244911 creator A5059221192 @default.
• W2012244911 creator A5090428657 @default.
• W2012244911 date "2012-06-01" @default.
• W2012244911 modified "2023-09-25" @default.
• W2012244911 title "Acceleration of gene transfection efficiency in neuroblastoma cells through polyethyleneimine/poly(methyl methacrylate) core-shell magnetic nanoparticles" @default.
• W2012244911 cites W1979866703 @default.
• W2012244911 cites W2029129350 @default.
• W2012244911 cites W2034944596 @default.
• W2012244911 cites W2057999099 @default.
• W2012244911 cites W2062787494 @default.
• W2012244911 cites W2073293452 @default.
• W2012244911 cites W2076752324 @default.
• W2012244911 cites W2080665606 @default.
• W2012244911 cites W2087968728 @default.
• W2012244911 cites W2092446347 @default.
• W2012244911 cites W2094146162 @default.
• W2012244911 cites W2124560116 @default.
• W2012244911 cites W2132005699 @default.
• W2012244911 cites W2141419526 @default.
• W2012244911 cites W2143827549 @default.
• W2012244911 cites W2152594308 @default.
• W2012244911 cites W2153042097 @default.
• W2012244911 cites W2157926595 @default.
• W2012244911 cites W2161757353 @default.
• W2012244911 cites W2167397010 @default.
• W2012244911 cites W2170313809 @default.
• W2012244911 cites W2180851115 @default.
• W2012244911 doi "https://doi.org/10.2147/ijn.s32311" @default.
• W2012244911 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3373300" @default.
• W2012244911 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22701321" @default.
• W2012244911 hasPublicationYear "2012" @default.
• W2012244911 type Work @default.
• W2012244911 sameAs 2012244911 @default.
• W2012244911 citedByCount "2" @default.
• W2012244911 countsByYear W20122449112017 @default.
• W2012244911 countsByYear W20122449112020 @default.
• W2012244911 crossrefType "journal-article" @default.
• W2012244911 hasAuthorship W2012244911A5024051438 @default.
• W2012244911 hasAuthorship W2012244911A5039159399 @default.
• W2012244911 hasAuthorship W2012244911A5049224975 @default.
• W2012244911 hasAuthorship W2012244911A5051111931 @default.
• W2012244911 hasAuthorship W2012244911A5056916296 @default.
• W2012244911 hasAuthorship W2012244911A5059221192 @default.
• W2012244911 hasAuthorship W2012244911A5090428657 @default.
• W2012244911 hasBestOaLocation W20122449111 @default.
• W2012244911 hasConcept C104317684 @default.
• W2012244911 hasConcept C12554922 @default.
• W2012244911 hasConcept C132050475 @default.
• W2012244911 hasConcept C135983454 @default.
• W2012244911 hasConcept C15083742 @default.
• W2012244911 hasConcept C155672457 @default.
• W2012244911 hasConcept C171250308 @default.
• W2012244911 hasConcept C178790620 @default.
• W2012244911 hasConcept C185592680 @default.
• W2012244911 hasConcept C192562407 @default.
• W2012244911 hasConcept C199002037 @default.
• W2012244911 hasConcept C44228677 @default.
• W2012244911 hasConcept C521977710 @default.
• W2012244911 hasConcept C54009773 @default.
• W2012244911 hasConcept C55493867 @default.
• W2012244911 hasConcept C86181022 @default.
• W2012244911 hasConcept C86803240 @default.
• W2012244911 hasConcept C87023908 @default.
• W2012244911 hasConceptScore W2012244911C104317684 @default.
• W2012244911 hasConceptScore W2012244911C12554922 @default.
• W2012244911 hasConceptScore W2012244911C132050475 @default.
• W2012244911 hasConceptScore W2012244911C135983454 @default.
• W2012244911 hasConceptScore W2012244911C15083742 @default.
• W2012244911 hasConceptScore W2012244911C155672457 @default.
• W2012244911 hasConceptScore W2012244911C171250308 @default.
• W2012244911 hasConceptScore W2012244911C178790620 @default.
• W2012244911 hasConceptScore W2012244911C185592680 @default.
• W2012244911 hasConceptScore W2012244911C192562407 @default.
• W2012244911 hasConceptScore W2012244911C199002037 @default.
• W2012244911 hasConceptScore W2012244911C44228677 @default.
• W2012244911 hasConceptScore W2012244911C521977710 @default.
• W2012244911 hasConceptScore W2012244911C54009773 @default.
• W2012244911 hasConceptScore W2012244911C55493867 @default.
• W2012244911 hasConceptScore W2012244911C86181022 @default.
• W2012244911 hasConceptScore W2012244911C86803240 @default.
• W2012244911 hasConceptScore W2012244911C87023908 @default.
• W2012244911 hasLocation W20122449111 @default.
• W2012244911 hasLocation W20122449112 @default.
• W2012244911 hasLocation W20122449113 @default.
• W2012244911 hasLocation W20122449114 @default.
• W2012244911 hasOpenAccess W2012244911 @default.
• W2012244911 hasPrimaryLocation W20122449111 @default.
• W2012244911 hasRelatedWork W1541746576 @default.
• W2012244911 hasRelatedWork W1993198130 @default.
• W2012244911 hasRelatedWork W2075559965 @default.
• W2012244911 hasRelatedWork W2076107990 @default.

• next