Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012245873> ?p ?o ?g. }
- W2012245873 endingPage "291" @default.
- W2012245873 startingPage "283" @default.
- W2012245873 abstract "A fundamental challenge in basic and applied biology is to reprogram cells with improved or novel traits on a genomic scale. However, the current ability to reprogram a cell on the genome scale is limited to bacterial cells. Here, we report RNA interference (RNAi)-assisted genome evolution (RAGE) as a generally applicable method for genome-scale engineering in the yeast Saccharomyces cerevisiae. Through iterative cycles of creating a library of RNAi induced reduction-of-function mutants coupled with high throughput screening or selection, RAGE can continuously improve target trait(s) by accumulating multiplex beneficial genetic modifications in an evolving yeast genome. To validate the RNAi library constructed with yeast genomic DNA and convergent-promoter expression cassette, we demonstrated RNAi screening in Saccharomyces cerevisiae for the first time by identifying two known and three novel suppressors of a telomerase-deficient mutation yku70Δ. We then showed the application of RAGE for improved acetic acid tolerance, a key trait for microbial production of chemicals and fuels. Three rounds of iterative RNAi screening led to the identification of three gene knockdown targets that acted synergistically to confer an engineered yeast strain with substantially improved acetic acid tolerance. RAGE should greatly accelerate the design and evolution of organisms with desired traits and provide new insights on genome structure, function, and evolution." @default.
- W2012245873 created "2016-06-24" @default.
- W2012245873 creator A5014465828 @default.
- W2012245873 creator A5040358433 @default.
- W2012245873 creator A5051495137 @default.
- W2012245873 creator A5064597661 @default.
- W2012245873 date "2014-05-06" @default.
- W2012245873 modified "2023-10-17" @default.
- W2012245873 title "RNAi-Assisted Genome Evolution in <i>Saccharomyces cerevisiae</i> for Complex Phenotype Engineering" @default.
- W2012245873 cites W1499220571 @default.
- W2012245873 cites W1530814332 @default.
- W2012245873 cites W1647075334 @default.
- W2012245873 cites W1965541518 @default.
- W2012245873 cites W1967210731 @default.
- W2012245873 cites W1968410036 @default.
- W2012245873 cites W1971411221 @default.
- W2012245873 cites W1971962952 @default.
- W2012245873 cites W1975255125 @default.
- W2012245873 cites W1979705207 @default.
- W2012245873 cites W1980658348 @default.
- W2012245873 cites W1982969769 @default.
- W2012245873 cites W1983741377 @default.
- W2012245873 cites W1996568337 @default.
- W2012245873 cites W1998128193 @default.
- W2012245873 cites W2000004958 @default.
- W2012245873 cites W2017642141 @default.
- W2012245873 cites W2018324060 @default.
- W2012245873 cites W2021905258 @default.
- W2012245873 cites W2023382748 @default.
- W2012245873 cites W2034510612 @default.
- W2012245873 cites W2038591876 @default.
- W2012245873 cites W2042931566 @default.
- W2012245873 cites W2043828851 @default.
- W2012245873 cites W2044954993 @default.
- W2012245873 cites W2058918320 @default.
- W2012245873 cites W2061184966 @default.
- W2012245873 cites W2061725821 @default.
- W2012245873 cites W2064815984 @default.
- W2012245873 cites W2066618807 @default.
- W2012245873 cites W2080537888 @default.
- W2012245873 cites W2088631531 @default.
- W2012245873 cites W2090041076 @default.
- W2012245873 cites W2095722471 @default.
- W2012245873 cites W2097131232 @default.
- W2012245873 cites W2102484627 @default.
- W2012245873 cites W2102957972 @default.
- W2012245873 cites W2103385859 @default.
- W2012245873 cites W2103602723 @default.
- W2012245873 cites W2115420193 @default.
- W2012245873 cites W2121977433 @default.
- W2012245873 cites W2122828981 @default.
- W2012245873 cites W2127073167 @default.
- W2012245873 cites W2130980587 @default.
- W2012245873 cites W2133102806 @default.
- W2012245873 cites W2137315132 @default.
- W2012245873 cites W2137406125 @default.
- W2012245873 cites W2140948740 @default.
- W2012245873 cites W2142437744 @default.
- W2012245873 cites W2144654038 @default.
- W2012245873 cites W2144779133 @default.
- W2012245873 cites W2145265114 @default.
- W2012245873 cites W2162261435 @default.
- W2012245873 cites W2169805130 @default.
- W2012245873 cites W2319568809 @default.
- W2012245873 cites W2419206391 @default.
- W2012245873 cites W4211039187 @default.
- W2012245873 cites W93382468 @default.
- W2012245873 doi "https://doi.org/10.1021/sb500074a" @default.
- W2012245873 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24758359" @default.
- W2012245873 hasPublicationYear "2014" @default.
- W2012245873 type Work @default.
- W2012245873 sameAs 2012245873 @default.
- W2012245873 citedByCount "68" @default.
- W2012245873 countsByYear W20122458732014 @default.
- W2012245873 countsByYear W20122458732015 @default.
- W2012245873 countsByYear W20122458732016 @default.
- W2012245873 countsByYear W20122458732017 @default.
- W2012245873 countsByYear W20122458732018 @default.
- W2012245873 countsByYear W20122458732019 @default.
- W2012245873 countsByYear W20122458732020 @default.
- W2012245873 countsByYear W20122458732021 @default.
- W2012245873 countsByYear W20122458732022 @default.
- W2012245873 countsByYear W20122458732023 @default.
- W2012245873 crossrefType "journal-article" @default.
- W2012245873 hasAuthorship W2012245873A5014465828 @default.
- W2012245873 hasAuthorship W2012245873A5040358433 @default.
- W2012245873 hasAuthorship W2012245873A5051495137 @default.
- W2012245873 hasAuthorship W2012245873A5064597661 @default.
- W2012245873 hasConcept C104317684 @default.
- W2012245873 hasConcept C141231307 @default.
- W2012245873 hasConcept C144501496 @default.
- W2012245873 hasConcept C145290725 @default.
- W2012245873 hasConcept C166703698 @default.
- W2012245873 hasConcept C173396325 @default.
- W2012245873 hasConcept C2777576037 @default.
- W2012245873 hasConcept C54355233 @default.
- W2012245873 hasConcept C67705224 @default.
- W2012245873 hasConcept C70721500 @default.