FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012247587> ?p ?o ?g. }

• W2012247587 endingPage "152" @default.
• W2012247587 startingPage "145" @default.
• W2012247587 abstract "A recombinant adenovirus encoding human endostatin gene, E10A, has finished phase II trials for head and neck cancer. However, the rigid storage temperature (-80°C) and the toxicity of glycerol in the E10A liquid preparation limited its clinical application. In this study, lyophilization was applied to develop a stable E10A lyophilized powder without glycerol that is able to maintain biological activity at 4°C and suitable for intravenous administration. The E10A lyophilized formulations composed of nontoxic and already clinically used excipients were characterized in terms of the pH change during freezing, the eutectic melting temperature (T(eu)) and the collapse temperature (T(c)). Freeze thawing tests were carried out to examine the protective effect of various excipients during freezing. Mannitol and its combinations with sucrose or inulin showed effective protection of E10A. The E10A lyophilized powders were analyzed by particle size measurement, residual humidity quantification, infectivity assay and gene expression level. An optimized formulation (formulation I1) yielded a good recovery of 76% of the starting infectivity after lyophilization and 89% of the original infectivity after storage at 4°C for 180 days. Also the gene expression capability of E10A in formulation I1 was maintained after lyophilization. In addition, it was found that the matrix of amorphous excipients, mannitol combinations with sucrose or inulin, was indispensible in protecting E10A against the stress of freezing and dehydration. Hereby, the E10A lyophilized powder with eliminated glycerol toxicity and improved stability could enhance the applicability of E10A for cancer gene therapy through intravenous administration." @default.
• W2012247587 created "2016-06-24" @default.
• W2012247587 creator A5004825388 @default.
• W2012247587 creator A5013852704 @default.
• W2012247587 creator A5018568356 @default.
• W2012247587 creator A5027791346 @default.
• W2012247587 creator A5028840870 @default.
• W2012247587 creator A5052579493 @default.
• W2012247587 creator A5074002910 @default.
• W2012247587 creator A5089553016 @default.
• W2012247587 date "2012-05-01" @default.
• W2012247587 modified "2023-09-27" @default.
• W2012247587 title "Investigation on formulation and preparation of adenovirus encoding human endostatin lyophilized powders" @default.
• W2012247587 cites W139461521 @default.
• W2012247587 cites W1525207856 @default.
• W2012247587 cites W1541087680 @default.
• W2012247587 cites W1974470466 @default.
• W2012247587 cites W1978778989 @default.
• W2012247587 cites W2021743560 @default.
• W2012247587 cites W2029492652 @default.
• W2012247587 cites W2030181518 @default.
• W2012247587 cites W2032755224 @default.
• W2012247587 cites W2039314453 @default.
• W2012247587 cites W2044985875 @default.
• W2012247587 cites W2059564760 @default.
• W2012247587 cites W2066662804 @default.
• W2012247587 cites W2071930478 @default.
• W2012247587 cites W2072856983 @default.
• W2012247587 cites W2073208004 @default.
• W2012247587 cites W2075771522 @default.
• W2012247587 cites W2075780259 @default.
• W2012247587 cites W2107882642 @default.
• W2012247587 cites W2120826143 @default.
• W2012247587 cites W2128052373 @default.
• W2012247587 cites W2136546144 @default.
• W2012247587 cites W2139354653 @default.
• W2012247587 cites W2155886309 @default.
• W2012247587 cites W2161001526 @default.
• W2012247587 cites W2189950502 @default.
• W2012247587 cites W57312166 @default.
• W2012247587 doi "https://doi.org/10.1016/j.ijpharm.2011.12.056" @default.
• W2012247587 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22234038" @default.
• W2012247587 hasPublicationYear "2012" @default.
• W2012247587 type Work @default.
• W2012247587 sameAs 2012247587 @default.
• W2012247587 citedByCount "12" @default.
• W2012247587 countsByYear W20122475872015 @default.
• W2012247587 countsByYear W20122475872016 @default.
• W2012247587 countsByYear W20122475872019 @default.
• W2012247587 countsByYear W20122475872020 @default.
• W2012247587 countsByYear W20122475872021 @default.
• W2012247587 countsByYear W20122475872023 @default.
• W2012247587 crossrefType "journal-article" @default.
• W2012247587 hasAuthorship W2012247587A5004825388 @default.
• W2012247587 hasAuthorship W2012247587A5013852704 @default.
• W2012247587 hasAuthorship W2012247587A5018568356 @default.
• W2012247587 hasAuthorship W2012247587A5027791346 @default.
• W2012247587 hasAuthorship W2012247587A5028840870 @default.
• W2012247587 hasAuthorship W2012247587A5052579493 @default.
• W2012247587 hasAuthorship W2012247587A5074002910 @default.
• W2012247587 hasAuthorship W2012247587A5089553016 @default.
• W2012247587 hasConcept C106358424 @default.
• W2012247587 hasConcept C159047783 @default.
• W2012247587 hasConcept C179933525 @default.
• W2012247587 hasConcept C185592680 @default.
• W2012247587 hasConcept C196843134 @default.
• W2012247587 hasConcept C2522874641 @default.
• W2012247587 hasConcept C2777239854 @default.
• W2012247587 hasConcept C2778445172 @default.
• W2012247587 hasConcept C2778597767 @default.
• W2012247587 hasConcept C2779587474 @default.
• W2012247587 hasConcept C2779742124 @default.
• W2012247587 hasConcept C2780881558 @default.
• W2012247587 hasConcept C43617362 @default.
• W2012247587 hasConcept C55493867 @default.
• W2012247587 hasConcept C68528507 @default.
• W2012247587 hasConcept C71924100 @default.
• W2012247587 hasConcept C84199377 @default.
• W2012247587 hasConcept C86803240 @default.
• W2012247587 hasConcept C95444343 @default.
• W2012247587 hasConcept C98274493 @default.
• W2012247587 hasConceptScore W2012247587C106358424 @default.
• W2012247587 hasConceptScore W2012247587C159047783 @default.
• W2012247587 hasConceptScore W2012247587C179933525 @default.
• W2012247587 hasConceptScore W2012247587C185592680 @default.
• W2012247587 hasConceptScore W2012247587C196843134 @default.
• W2012247587 hasConceptScore W2012247587C2522874641 @default.
• W2012247587 hasConceptScore W2012247587C2777239854 @default.
• W2012247587 hasConceptScore W2012247587C2778445172 @default.
• W2012247587 hasConceptScore W2012247587C2778597767 @default.
• W2012247587 hasConceptScore W2012247587C2779587474 @default.
• W2012247587 hasConceptScore W2012247587C2779742124 @default.
• W2012247587 hasConceptScore W2012247587C2780881558 @default.
• W2012247587 hasConceptScore W2012247587C43617362 @default.
• W2012247587 hasConceptScore W2012247587C55493867 @default.
• W2012247587 hasConceptScore W2012247587C68528507 @default.
• W2012247587 hasConceptScore W2012247587C71924100 @default.
• W2012247587 hasConceptScore W2012247587C84199377 @default.

• next