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- W2012247978 abstract "An absolute quantification method for the N-myc gene copy number of neuroblastoma specimens was established by applying the competitive polymerase chain reaction (cPCR). The competitor plasmid (pZH2) lacking an MluI site in the exon 2 was constructed to distinguish two product species amplified from genomic DNA and the competitor plasmid. By using this cPCR system, we could obtain qualitative results within 1 day, i.e. amplified or unamplified, and quantitative results by using radiolabelled nucleotides within 4 days. The copy numbers of N-myc in 47 neuroblastoma specimens by cPCR correlated well with those by Southern hybridization (r = 0.85). We conclude that cPCR is a simple and rapid method, requires only a small amount (200 ng) of sample DNA, and is expected to be used for prognostic evaluation in neuroblastomas." @default.
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- W2012247978 date "1996-01-01" @default.
- W2012247978 modified "2023-09-27" @default.
- W2012247978 title "Competitive Polymerase Chain Reaction for the Quantification of N-<i>myc</i> Gene Copy Number in Neuroblastoma" @default.
- W2012247978 doi "https://doi.org/10.1159/000217988" @default.
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