FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012249620> ?p ?o ?g. }

• W2012249620 endingPage "46" @default.
• W2012249620 startingPage "36" @default.
• W2012249620 abstract "In this report, a combined imaging and fluorescence correlation spectroscopy (FCS) method is described and its ability to characterize microsecond fluctuations in the fluorescence emission of a sample is demonstrated. A sample scanning laser confocal microscope is operated in the customary way while recording the time that each photon is detected with a time resolution of 50 ns using a low-cost counting board. The serial data stream of photon detection times allows access to fluorescence signal fluctuations that can be used to characterize dynamics using correlation methods. The same data stream is used to generate images of the sample. Using the technique, we demonstrate that it is possible to characterize the kinetics of transitions to and from nonemitting or dark states of the fluorescent dyes DiIC16 and ATTO 520. Results are similar to, but deviate slightly from, a model that has been frequently used for extracting singlet-triplet: conversion rates using conventional solution-based FCS. Like conventional FCS, the concentration, or in our case the areal density of coverage, of fluorescent species can also be obtained. Many single-molecule fluorescence experiments aim to extract kinetics from intensity trajectories; this method may be used as a rapid and convenient technique for characterization of surface-linked or thin-film samples prior to performing the more time and effort intensive single-molecule measurements. Besides the capacity to measure photophysical phenomena, the surface-sensitive FCS method could also be applied for measuring conformational changes or interaction kinetics for species immobilized on a surface. One possible scenario is measurements of the frequency and duration of association of ligand-receptor pairs where a fluorescently labeled component is freely diffusing and the other is surface immobilized. Given that microarrays of custom-designed, surface-immobilized peptides and nucleic acids are now readily available, the ability to sensitively measure association and dissociation rates of the surface-linked species with a freely diffusing species could be a useful extension to what has already become an extremely important tool for characterizing the interactions of biomolecules." @default.
• W2012249620 created "2016-06-24" @default.
• W2012249620 creator A5005428764 @default.
• W2012249620 creator A5034296702 @default.
• W2012249620 creator A5079985074 @default.
• W2012249620 date "2004-11-19" @default.
• W2012249620 modified "2023-09-23" @default.
• W2012249620 title "Scanning Fluorescence Correlation Spectroscopy: A Tool for Probing Microsecond Dynamics of Surface-Bound Fluorescent Species" @default.
• W2012249620 cites W1964048941 @default.
• W2012249620 cites W1972595608 @default.
• W2012249620 cites W1973948015 @default.
• W2012249620 cites W1974909861 @default.
• W2012249620 cites W1976786825 @default.
• W2012249620 cites W1977471961 @default.
• W2012249620 cites W1979623029 @default.
• W2012249620 cites W1981765336 @default.
• W2012249620 cites W1985230131 @default.
• W2012249620 cites W1987824698 @default.
• W2012249620 cites W1988328429 @default.
• W2012249620 cites W1994333574 @default.
• W2012249620 cites W2001274423 @default.
• W2012249620 cites W2013044650 @default.
• W2012249620 cites W2014603022 @default.
• W2012249620 cites W2029370337 @default.
• W2012249620 cites W2035462222 @default.
• W2012249620 cites W2039009473 @default.
• W2012249620 cites W2040103756 @default.
• W2012249620 cites W2040140501 @default.
• W2012249620 cites W2042382390 @default.
• W2012249620 cites W2044213208 @default.
• W2012249620 cites W2049093445 @default.
• W2012249620 cites W2050423264 @default.
• W2012249620 cites W2058300904 @default.
• W2012249620 cites W2058308507 @default.
• W2012249620 cites W2060683792 @default.
• W2012249620 cites W2062515534 @default.
• W2012249620 cites W2063337375 @default.
• W2012249620 cites W2068696224 @default.
• W2012249620 cites W2073271190 @default.
• W2012249620 cites W2079009915 @default.
• W2012249620 cites W2086836596 @default.
• W2012249620 cites W2106674708 @default.
• W2012249620 cites W2129995310 @default.
• W2012249620 cites W2132192400 @default.
• W2012249620 cites W2137978643 @default.
• W2012249620 cites W2139798839 @default.
• W2012249620 cites W2148825507 @default.
• W2012249620 cites W2149627878 @default.
• W2012249620 cites W2495859430 @default.
• W2012249620 cites W3044583775 @default.
• W2012249620 doi "https://doi.org/10.1021/ac049010z" @default.
• W2012249620 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15623276" @default.
• W2012249620 hasPublicationYear "2004" @default.
• W2012249620 type Work @default.
• W2012249620 sameAs 2012249620 @default.
• W2012249620 citedByCount "34" @default.
• W2012249620 countsByYear W20122496202012 @default.
• W2012249620 countsByYear W20122496202014 @default.
• W2012249620 countsByYear W20122496202016 @default.
• W2012249620 countsByYear W20122496202021 @default.
• W2012249620 crossrefType "journal-article" @default.
• W2012249620 hasAuthorship W2012249620A5005428764 @default.
• W2012249620 hasAuthorship W2012249620A5034296702 @default.
• W2012249620 hasAuthorship W2012249620A5079985074 @default.
• W2012249620 hasConcept C113196181 @default.
• W2012249620 hasConcept C120665830 @default.
• W2012249620 hasConcept C121332964 @default.
• W2012249620 hasConcept C144618238 @default.
• W2012249620 hasConcept C147080431 @default.
• W2012249620 hasConcept C148898269 @default.
• W2012249620 hasConcept C158711907 @default.
• W2012249620 hasConcept C159317903 @default.
• W2012249620 hasConcept C185592680 @default.
• W2012249620 hasConcept C2781402376 @default.
• W2012249620 hasConcept C32891209 @default.
• W2012249620 hasConcept C34742353 @default.
• W2012249620 hasConcept C36038061 @default.
• W2012249620 hasConcept C43617362 @default.
• W2012249620 hasConcept C62520636 @default.
• W2012249620 hasConcept C67649825 @default.
• W2012249620 hasConcept C91881484 @default.
• W2012249620 hasConceptScore W2012249620C113196181 @default.
• W2012249620 hasConceptScore W2012249620C120665830 @default.
• W2012249620 hasConceptScore W2012249620C121332964 @default.
• W2012249620 hasConceptScore W2012249620C144618238 @default.
• W2012249620 hasConceptScore W2012249620C147080431 @default.
• W2012249620 hasConceptScore W2012249620C148898269 @default.
• W2012249620 hasConceptScore W2012249620C158711907 @default.
• W2012249620 hasConceptScore W2012249620C159317903 @default.
• W2012249620 hasConceptScore W2012249620C185592680 @default.
• W2012249620 hasConceptScore W2012249620C2781402376 @default.
• W2012249620 hasConceptScore W2012249620C32891209 @default.
• W2012249620 hasConceptScore W2012249620C34742353 @default.
• W2012249620 hasConceptScore W2012249620C36038061 @default.
• W2012249620 hasConceptScore W2012249620C43617362 @default.
• W2012249620 hasConceptScore W2012249620C62520636 @default.
• W2012249620 hasConceptScore W2012249620C67649825 @default.
• W2012249620 hasConceptScore W2012249620C91881484 @default.

• next