FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012251384> ?p ?o ?g. }

• W2012251384 endingPage "499" @default.
• W2012251384 startingPage "490" @default.
• W2012251384 abstract "SVMPA, a mutant of Sindbis virus derived by serial passage on Aedes albopictus mosquito cells maintained after infection in the presence of mycophenolic acid (MPA), is resistant not only to MPA but also to ribavirin. Both of these compounds inhibit the synthesis of GMP and thereby reduce the level of GTP. We had suggested earlier that SVMPA had become resistant to MPA because it coded for an altered RNA guanylyltransferase enzyme with an increased affinity for GTP, enabling it to replicate in cells with reduced levels of GTP. We now report that the MPA-resistant phenotype of SVMPA has been mapped to the coding region for the nonstructural viral protein, nsP1. By replacing the nucleotide sequence between 88 and 1404 of the infectious clone of Sindbis virus (i.e., the Toto 1101 plasmid) with the corresponding sequence from SVMPA cDNA, we were able to generate recombinant Sindbis virus expressing the drug-resistant phenotype. SVMPA has three base substitutions in the region between nucleotides 88 and 1404 which lead to predicted amino acid changes in the Sindbis virus nsP1 protein: the replacement of Gln at residue 21 by Lys, Ser at residue 23 by Asn, and Val at residue 302 by Met. These results, taken together with previous data from our laboratory associating the RNA methyltransferase with nsP1, (1) are consistent with the idea that an alteration of the RNA guanylyltransferase is responsible for the MPA-resistant phenotype and (2) support the idea that an important function of nsP1 relates to the modification of the 5′ terminus of the Sindbis virus mRNAs." @default.
• W2012251384 created "2016-06-24" @default.
• W2012251384 creator A5004231173 @default.
• W2012251384 creator A5090331176 @default.
• W2012251384 date "1991-04-01" @default.
• W2012251384 modified "2023-09-28" @default.
• W2012251384 title "Mutations that confer resistance to mycophenolic acid and ribavirin on sindbis virus map to the nonstructural protein nsP1" @default.
• W2012251384 cites W1484881742 @default.
• W2012251384 cites W1489499808 @default.
• W2012251384 cites W1495237126 @default.
• W2012251384 cites W1498496591 @default.
• W2012251384 cites W1532353118 @default.
• W2012251384 cites W1552295026 @default.
• W2012251384 cites W1581094257 @default.
• W2012251384 cites W1628437610 @default.
• W2012251384 cites W1870498429 @default.
• W2012251384 cites W1912010573 @default.
• W2012251384 cites W1970657748 @default.
• W2012251384 cites W1971734995 @default.
• W2012251384 cites W1975744697 @default.
• W2012251384 cites W1981806274 @default.
• W2012251384 cites W1989314579 @default.
• W2012251384 cites W1990459486 @default.
• W2012251384 cites W2022584033 @default.
• W2012251384 cites W2022765393 @default.
• W2012251384 cites W2023413072 @default.
• W2012251384 cites W2044524906 @default.
• W2012251384 cites W2046828533 @default.
• W2012251384 cites W2049449836 @default.
• W2012251384 cites W2072343418 @default.
• W2012251384 cites W2073266170 @default.
• W2012251384 cites W2090822075 @default.
• W2012251384 cites W2103800225 @default.
• W2012251384 cites W2113806981 @default.
• W2012251384 cites W2119889845 @default.
• W2012251384 cites W4238099995 @default.
• W2012251384 doi "https://doi.org/10.1016/0042-6822(91)90881-b" @default.
• W2012251384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1826574" @default.
• W2012251384 hasPublicationYear "1991" @default.
• W2012251384 type Work @default.
• W2012251384 sameAs 2012251384 @default.
• W2012251384 citedByCount "107" @default.
• W2012251384 countsByYear W20122513842012 @default.
• W2012251384 countsByYear W20122513842013 @default.
• W2012251384 countsByYear W20122513842014 @default.
• W2012251384 countsByYear W20122513842015 @default.
• W2012251384 countsByYear W20122513842016 @default.
• W2012251384 countsByYear W20122513842017 @default.
• W2012251384 countsByYear W20122513842018 @default.
• W2012251384 countsByYear W20122513842019 @default.
• W2012251384 countsByYear W20122513842020 @default.
• W2012251384 countsByYear W20122513842021 @default.
• W2012251384 countsByYear W20122513842022 @default.
• W2012251384 countsByYear W20122513842023 @default.
• W2012251384 crossrefType "journal-article" @default.
• W2012251384 hasAuthorship W2012251384A5004231173 @default.
• W2012251384 hasAuthorship W2012251384A5090331176 @default.
• W2012251384 hasConcept C104317684 @default.
• W2012251384 hasConcept C153911025 @default.
• W2012251384 hasConcept C159047783 @default.
• W2012251384 hasConcept C2522874641 @default.
• W2012251384 hasConcept C2777343345 @default.
• W2012251384 hasConcept C2777474056 @default.
• W2012251384 hasConcept C55493867 @default.
• W2012251384 hasConcept C67705224 @default.
• W2012251384 hasConcept C86803240 @default.
• W2012251384 hasConceptScore W2012251384C104317684 @default.
• W2012251384 hasConceptScore W2012251384C153911025 @default.
• W2012251384 hasConceptScore W2012251384C159047783 @default.
• W2012251384 hasConceptScore W2012251384C2522874641 @default.
• W2012251384 hasConceptScore W2012251384C2777343345 @default.
• W2012251384 hasConceptScore W2012251384C2777474056 @default.
• W2012251384 hasConceptScore W2012251384C55493867 @default.
• W2012251384 hasConceptScore W2012251384C67705224 @default.
• W2012251384 hasConceptScore W2012251384C86803240 @default.
• W2012251384 hasIssue "2" @default.
• W2012251384 hasLocation W20122513841 @default.
• W2012251384 hasLocation W20122513842 @default.
• W2012251384 hasOpenAccess W2012251384 @default.
• W2012251384 hasPrimaryLocation W20122513841 @default.
• W2012251384 hasRelatedWork W1578192730 @default.
• W2012251384 hasRelatedWork W1933588764 @default.
• W2012251384 hasRelatedWork W2009750207 @default.
• W2012251384 hasRelatedWork W2031415975 @default.
• W2012251384 hasRelatedWork W2086884780 @default.
• W2012251384 hasRelatedWork W2110629857 @default.
• W2012251384 hasRelatedWork W2161361622 @default.
• W2012251384 hasRelatedWork W2365294297 @default.
• W2012251384 hasRelatedWork W3127272920 @default.
• W2012251384 hasRelatedWork W4297064195 @default.
• W2012251384 hasVolume "181" @default.
• W2012251384 isParatext "false" @default.
• W2012251384 isRetracted "false" @default.
• W2012251384 magId "2012251384" @default.
• W2012251384 workType "article" @default.