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- W2012256457 abstract "Glutamate decarboxylase (GAD) (EC 4.1.1.15) catalyzes decarboxylation of glutamic acid to produce gammaaminobutyric acid (GABA). A putative gad gene (tk1814) from an archaeon Thermococcus kodakaraensis KOD1 was cloned and transformed into Escherichia coli to produce a bulk amount of recombinant GAD. Activity of the purified GAD was optimal at 90°C and pH 8.0. Optimal concentration of substrate for conversion into gamma-aminobutyric acid by recombinant GAD was 50 mM monosodium glutamate. Recombinant GAD was confirmed to be monomeric, and its activity was greatly inhibited by various salts such as sodium chloride, Tris-HCl, and sodium phosphate. Km, Vmax, and Kcat values were 9.92 mM, 153.8 μmol min−1 mg−1, and 6.613×103 min−1 respectively." @default.
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- W2012256457 date "2012-04-01" @default.
- W2012256457 modified "2023-10-09" @default.
- W2012256457 title "Overexpression and characterization of recombinant glutamate decarboxylase from Thermococcus kodakaraensis KOD1" @default.
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- W2012256457 doi "https://doi.org/10.1007/s13765-012-1006-z" @default.
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