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- W2012257102 abstract "Summary: A method is described for the determination of vigabatrin in 50 μl of plasma by isocratic high-performance liquid chromatography using fluorescence detection. The procedure involves protein precipitation with methanol followed by precolumn derivatisation with o-phthaldialdehyde reagent. Separation of the derivatised vigabatrin was achieved on a Microsorb C18 column using a mobile phase of 10 mM orthophosphoric acid:acetonitrile:methanol (6:3:1) at a flow rate of 2.0 ml/min. Assay time is 15 min and chromatograms show no interference from commonly coadministered anticonvulsant drugs. The total analytical error within the range of 0.85–85 μg/ml was found to be 7.6% with the within-replicates error of 2.76%. The minimum detection limit was 0.08 μg/ml and the minimum quantitation limit was 0.54 μg/ml." @default.
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- W2012257102 date "1991-05-01" @default.
- W2012257102 modified "2023-09-26" @default.
- W2012257102 title "Determination of Vigabatrin in Plasma by Reversed-Phase High-Performance Liquid Chromatography" @default.
- W2012257102 doi "https://doi.org/10.1097/00007691-199105000-00011" @default.
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