Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012257985> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2012257985 endingPage "451" @default.
- W2012257985 startingPage "448" @default.
- W2012257985 abstract "LytC, one of the major autolysins from the human pathogen Streptococcus pneumoniae, has been crystallized as needles by the hanging-drop technique using 10%(w/v) PEG 3350 as precipitant and 10 mM HEPES pH 7.5. LytC crystals were quickly soaked in mother liquor containing 2 mM of the complex Gd-HPDO3A to produce derivatized crystals (LytC(Gd-HPDO3A)). Both native LytC and isomorphous LytC(Gd-HPDO3A) crystals were flash-cooled in a nitrogen flow at 120 K prior to X-ray data collection using an in-house Enraf-Nonius rotating-anode generator (lambda = 1.5418 A) and a MAR345 imaging-plate detector. In both cases, good-quality diffraction patterns were obtained at high resolution. LytC(Gd-HPDO3A) crystals allowed the collection of a SAD X-ray data set to 2.6 A resolution indexed in terms of a P2(1) monoclinic unit cell with parameters a = 59.37, b = 67.16, c = 78.85 A, beta = 105.69 degrees . The anomalous Patterson map allowed the identification of one heavy-atom binding site, which was sufficient for the calculation of an interpretable anomalous map at 2.6 A resolution." @default.
- W2012257985 created "2016-06-24" @default.
- W2012257985 creator A5021295789 @default.
- W2012257985 creator A5025745736 @default.
- W2012257985 creator A5042064315 @default.
- W2012257985 creator A5060687832 @default.
- W2012257985 creator A5068704164 @default.
- W2012257985 creator A5081288634 @default.
- W2012257985 creator A5082779361 @default.
- W2012257985 date "2010-03-31" @default.
- W2012257985 modified "2023-10-17" @default.
- W2012257985 title "Crystallization of the pneumococcal autolysin LytC: in-house phasing using novel lanthanide complexes" @default.
- W2012257985 cites W1969788984 @default.
- W2012257985 cites W1990607884 @default.
- W2012257985 cites W1999388517 @default.
- W2012257985 cites W2001641653 @default.
- W2012257985 cites W2012077135 @default.
- W2012257985 cites W2014694459 @default.
- W2012257985 cites W2043574444 @default.
- W2012257985 cites W2050738483 @default.
- W2012257985 cites W2070016337 @default.
- W2012257985 cites W2077728846 @default.
- W2012257985 cites W2098116539 @default.
- W2012257985 cites W2113326101 @default.
- W2012257985 cites W2131350133 @default.
- W2012257985 cites W2138666971 @default.
- W2012257985 doi "https://doi.org/10.1107/s1744309110006081" @default.
- W2012257985 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2852341" @default.
- W2012257985 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20383019" @default.
- W2012257985 hasPublicationYear "2010" @default.
- W2012257985 type Work @default.
- W2012257985 sameAs 2012257985 @default.
- W2012257985 citedByCount "2" @default.
- W2012257985 countsByYear W20122579852013 @default.
- W2012257985 countsByYear W20122579852021 @default.
- W2012257985 crossrefType "journal-article" @default.
- W2012257985 hasAuthorship W2012257985A5021295789 @default.
- W2012257985 hasAuthorship W2012257985A5025745736 @default.
- W2012257985 hasAuthorship W2012257985A5042064315 @default.
- W2012257985 hasAuthorship W2012257985A5060687832 @default.
- W2012257985 hasAuthorship W2012257985A5068704164 @default.
- W2012257985 hasAuthorship W2012257985A5081288634 @default.
- W2012257985 hasAuthorship W2012257985A5082779361 @default.
- W2012257985 hasBestOaLocation W20122579852 @default.
- W2012257985 hasConcept C115624301 @default.
- W2012257985 hasConcept C138268822 @default.
- W2012257985 hasConcept C154945302 @default.
- W2012257985 hasConcept C178790620 @default.
- W2012257985 hasConcept C185592680 @default.
- W2012257985 hasConcept C192562407 @default.
- W2012257985 hasConcept C203036418 @default.
- W2012257985 hasConcept C41008148 @default.
- W2012257985 hasConcept C61276311 @default.
- W2012257985 hasConcept C8010536 @default.
- W2012257985 hasConceptScore W2012257985C115624301 @default.
- W2012257985 hasConceptScore W2012257985C138268822 @default.
- W2012257985 hasConceptScore W2012257985C154945302 @default.
- W2012257985 hasConceptScore W2012257985C178790620 @default.
- W2012257985 hasConceptScore W2012257985C185592680 @default.
- W2012257985 hasConceptScore W2012257985C192562407 @default.
- W2012257985 hasConceptScore W2012257985C203036418 @default.
- W2012257985 hasConceptScore W2012257985C41008148 @default.
- W2012257985 hasConceptScore W2012257985C61276311 @default.
- W2012257985 hasConceptScore W2012257985C8010536 @default.
- W2012257985 hasIssue "4" @default.
- W2012257985 hasLocation W20122579851 @default.
- W2012257985 hasLocation W20122579852 @default.
- W2012257985 hasLocation W20122579853 @default.
- W2012257985 hasLocation W20122579854 @default.
- W2012257985 hasOpenAccess W2012257985 @default.
- W2012257985 hasPrimaryLocation W20122579851 @default.
- W2012257985 hasRelatedWork W1967994367 @default.
- W2012257985 hasRelatedWork W2031552096 @default.
- W2012257985 hasRelatedWork W2068766648 @default.
- W2012257985 hasRelatedWork W2076113927 @default.
- W2012257985 hasRelatedWork W2139360615 @default.
- W2012257985 hasRelatedWork W2151535407 @default.
- W2012257985 hasRelatedWork W2157727613 @default.
- W2012257985 hasRelatedWork W2373498662 @default.
- W2012257985 hasRelatedWork W2517621576 @default.
- W2012257985 hasRelatedWork W2998977823 @default.
- W2012257985 hasVolume "66" @default.
- W2012257985 isParatext "false" @default.
- W2012257985 isRetracted "false" @default.
- W2012257985 magId "2012257985" @default.
- W2012257985 workType "article" @default.