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- W2012258183 abstract "Using a cross-axis coil planet centrifuge, glycoproteins were separated from fermentation media of Morchella esculenta (L.) by high-speed counter-current chromatography. The performance of the apparatus was optimized with four standard proteins including pepsin, lysozyme, ovalbumin and hemoglobin and a polymer phase system composed of 12.5% (w/w) polyethylene glycol 8000 and 25% (w/w) potassium phosphate in distilled water at various pH values. Separations were performed by eluting the lower phosphate-rich phase at a flow-rate of 1.0 ml/min. Under the optimized conditions three glycoprotein components in Morchella esculenta (L.) were resolved within 6 h." @default.
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- W2012258183 date "2001-05-01" @default.
- W2012258183 modified "2023-09-27" @default.
- W2012258183 title "Counter-current chromatographic separation of glycoprotein components from Morchella esculenta (L.) with a polymer phase system by a cross-axis coil planet centrifuge" @default.
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- W2012258183 doi "https://doi.org/10.1016/s0021-9673(01)00665-3" @default.
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