FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012259686> ?p ?o ?g. }

• W2012259686 endingPage "2453" @default.
• W2012259686 startingPage "2444" @default.
• W2012259686 abstract "We have analyzed the dependence of actin filament sliding movement on the mode of myosin attachment to surfaces. Monoclonal antibodies (mAbs) that bind to three distinct sites were used to tether myosin to nitrocellulose-coated glass. One antibody reacts with an epitope on the regulatory light chain (LC2) located at the head-rod junction. The other two react with sites in the rod domain, one in the S2 region near the S2-LMM hinge, and the other at the C terminus of the myosin rod. This method of attachment provides a means of controlling the flexibility and density of myosin on the surface. Fast skeletal muscle myosin monomers were bound to the surfaces through the specific interaction with these mAbs, and the sliding movement of fluorescently labeled actin filaments was analyzed by video microscopy. Each of these antibodies produced stable myosin-coated surfaces that supported uniform motion of actin over the course of several hours. Attachment of myosin through the anti-S2 and anti-LMM mAbs yielded significantly higher velocities (10 microns/s at 30 degrees C) than attachment through anti-LC2 (4-5 microns/s at 30 degrees C). For each antibody, we observed a characteristic value of the myosin density for the onset of F-actin motion and a second critical density for velocity saturation. The specific mode of attachment influences the velocity of actin filaments and the characteristic surface density needed to support movement." @default.
• W2012259686 created "2016-06-24" @default.
• W2012259686 creator A5005435456 @default.
• W2012259686 creator A5007001742 @default.
• W2012259686 creator A5020873867 @default.
• W2012259686 creator A5042618944 @default.
• W2012259686 creator A5066230869 @default.
• W2012259686 date "1995-06-01" @default.
• W2012259686 modified "2023-10-17" @default.
• W2012259686 title "Flexibility of myosin attachment to surfaces influences F-actin motion" @default.
• W2012259686 cites W1427132408 @default.
• W2012259686 cites W1508733727 @default.
• W2012259686 cites W1526403580 @default.
• W2012259686 cites W1539885828 @default.
• W2012259686 cites W1545034454 @default.
• W2012259686 cites W1967496500 @default.
• W2012259686 cites W1975665006 @default.
• W2012259686 cites W1977461031 @default.
• W2012259686 cites W1979320189 @default.
• W2012259686 cites W1984684797 @default.
• W2012259686 cites W1988798258 @default.
• W2012259686 cites W1990379370 @default.
• W2012259686 cites W1993530325 @default.
• W2012259686 cites W1994466407 @default.
• W2012259686 cites W1996148284 @default.
• W2012259686 cites W2002684065 @default.
• W2012259686 cites W2026064166 @default.
• W2012259686 cites W2026836008 @default.
• W2012259686 cites W2030769440 @default.
• W2012259686 cites W2035703000 @default.
• W2012259686 cites W2052976658 @default.
• W2012259686 cites W2057866576 @default.
• W2012259686 cites W2059158601 @default.
• W2012259686 cites W2067750636 @default.
• W2012259686 cites W2069953906 @default.
• W2012259686 cites W2077999459 @default.
• W2012259686 cites W2079516004 @default.
• W2012259686 cites W2107236242 @default.
• W2012259686 cites W2119327575 @default.
• W2012259686 cites W2127917887 @default.
• W2012259686 cites W2140421284 @default.
• W2012259686 cites W2141586704 @default.
• W2012259686 cites W2218720641 @default.
• W2012259686 cites W2764833855 @default.
• W2012259686 doi "https://doi.org/10.1016/s0006-3495(95)80426-1" @default.
• W2012259686 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1282154" @default.
• W2012259686 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7544167" @default.
• W2012259686 hasPublicationYear "1995" @default.
• W2012259686 type Work @default.
• W2012259686 sameAs 2012259686 @default.
• W2012259686 citedByCount "48" @default.
• W2012259686 countsByYear W20122596862012 @default.
• W2012259686 countsByYear W20122596862013 @default.
• W2012259686 countsByYear W20122596862014 @default.
• W2012259686 countsByYear W20122596862015 @default.
• W2012259686 countsByYear W20122596862016 @default.
• W2012259686 countsByYear W20122596862017 @default.
• W2012259686 countsByYear W20122596862018 @default.
• W2012259686 countsByYear W20122596862019 @default.
• W2012259686 countsByYear W20122596862020 @default.
• W2012259686 countsByYear W20122596862022 @default.
• W2012259686 crossrefType "journal-article" @default.
• W2012259686 hasAuthorship W2012259686A5005435456 @default.
• W2012259686 hasAuthorship W2012259686A5007001742 @default.
• W2012259686 hasAuthorship W2012259686A5020873867 @default.
• W2012259686 hasAuthorship W2012259686A5042618944 @default.
• W2012259686 hasAuthorship W2012259686A5066230869 @default.
• W2012259686 hasBestOaLocation W20122596861 @default.
• W2012259686 hasConcept C12554922 @default.
• W2012259686 hasConcept C125705527 @default.
• W2012259686 hasConcept C14228908 @default.
• W2012259686 hasConcept C15954220 @default.
• W2012259686 hasConcept C159654299 @default.
• W2012259686 hasConcept C185592680 @default.
• W2012259686 hasConcept C18923293 @default.
• W2012259686 hasConcept C203014093 @default.
• W2012259686 hasConcept C542903549 @default.
• W2012259686 hasConcept C55493867 @default.
• W2012259686 hasConcept C6997183 @default.
• W2012259686 hasConcept C8010536 @default.
• W2012259686 hasConcept C8035138 @default.
• W2012259686 hasConcept C86803240 @default.
• W2012259686 hasConceptScore W2012259686C12554922 @default.
• W2012259686 hasConceptScore W2012259686C125705527 @default.
• W2012259686 hasConceptScore W2012259686C14228908 @default.
• W2012259686 hasConceptScore W2012259686C15954220 @default.
• W2012259686 hasConceptScore W2012259686C159654299 @default.
• W2012259686 hasConceptScore W2012259686C185592680 @default.
• W2012259686 hasConceptScore W2012259686C18923293 @default.
• W2012259686 hasConceptScore W2012259686C203014093 @default.
• W2012259686 hasConceptScore W2012259686C542903549 @default.
• W2012259686 hasConceptScore W2012259686C55493867 @default.
• W2012259686 hasConceptScore W2012259686C6997183 @default.
• W2012259686 hasConceptScore W2012259686C8010536 @default.
• W2012259686 hasConceptScore W2012259686C8035138 @default.
• W2012259686 hasConceptScore W2012259686C86803240 @default.
• W2012259686 hasIssue "6" @default.
• W2012259686 hasLocation W20122596861 @default.

• next