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- W2012292868 abstract "The cloned T brucei GAPDH gene was inserted within the B subtilis GAPDH gene, carried by pUC18. Upon transformation of B subtilis by this plasmid, not able to replicate in this host, the whole plasmid was inserted in the resident chromosome, presumably by a single recombination event between homologous, chromosomal and plasmid-borne sequences. The heterologous gene was expressed, as revealed by immunological reaction with monoclonal antibodies, recognizing specifically T brucei GAPDH. T brucei GAPDH, having little or no enzyme activity, comprises about 1.36% of cellular proteins. Peptide mapping showed that a fusion of a 7.5-kDA peptide had occurred to the N-terminal part of T brucei GAPDH. This fused protein is presumably the N-terminal part of B subtilis GAPDH, in agreement with the construction of the integrative plasmid." @default.
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- W2012292868 date "1992-02-01" @default.
- W2012292868 modified "2023-10-16" @default.
- W2012292868 title "Expression in Bacillus subtilis of the glycosomal glyceraldehyde-phosphate dehydrogenase gene from Trypanosoma brucei" @default.
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- W2012292868 doi "https://doi.org/10.1016/0300-9084(92)90037-f" @default.
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