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- W2012300612 abstract "A sensitive, low-temperature spectrophotometric method has been developed for independently estimating the quantities of cytochromes a and a3 in a single, hemoglobin- and/or myoglobin-contaminated tissue homogenate or cell suspension. The concentration dependence of cytochrome absorption at liquid nitrogen temperature and the intensification of the cytochrome absorbance due to light scattering by the frozen hyperosmotic sucrose solution were used for detecting small amounts of cytochromes. The absorbance due to contaminated hemoglobin and myoglobin was cancelled in the difference spectrum by pretreating the sample with CO:O2 (50:50) mixed gas. The resulting cytochrome a32+-CO compound was irreversibly photolyzed at −196°C, thus permitting us to obtain a fully reduced minus oxidized difference spectrum of the cytochromes. The method depends upon the non-Arrhenius behavior of carboxyhemoglobin and carboxymyoglobin which enables them to recombine with the CO molecule at the temperature of liquid nitrogen. Cytochrome a3 does not exhibit this extreme non-Arrhenius behavior and hence does not recombine at the low temperature. Two to five picomoles of cytochromes could be estimated by this method." @default.
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- W2012300612 date "1976-07-01" @default.
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- W2012300612 title "A sensitive method for the quantitative estimation of cytochromes a and a3 in tissues" @default.
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- W2012300612 doi "https://doi.org/10.1016/0003-2697(76)90313-4" @default.
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