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• W2012305727 abstract "The generation of free radicals under various conditions in the presence of methyl viologen (MV2+) was investigated in chloroplasts of wheat and lettuce by means of electron spin resonance (ESR) spectroscopy including spin trapping using 5,5-dimethyl-1-pyrroline-1-oxide (DMPO). An increase in the formation of different radical species, depending on the concentration of MV2+, the light intensity and the amount of chloroplasts, was observed. The monocation radical of MV2+ (MV) accumulated in the light under conditions of exhaustion of oxygen, and vanished in the dark. The disappearance of the ESR signal was strongly dependent on the amount of chloroplasts both for wheat and lettuce, indicating the transfer of electrons from MV to acceptors within the chloroplasts. NADPH stimulated and NADP+ decreased the formation of reactive oxygen species. The addition of the NADPH-specific enzyme glutathione reductase diminished the DMPOOH signal both in the absence and in the presence of MV2+ . This is explained by the restoration of the NADP+-pool by the action of the enzyme." @default.
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• W2012305727 date "1992-02-01" @default.
• W2012305727 modified "2023-10-01" @default.
• W2012305727 title "Free radical formation in chloroplasts. Methyl viologen action" @default.
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• W2012305727 doi "https://doi.org/10.1016/1011-1344(92)85042-s" @default.
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