FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012312915> ?p ?o ?g. }

• W2012312915 endingPage "e45393" @default.
• W2012312915 startingPage "e45393" @default.
• W2012312915 abstract "It is known that targeting of antigen to antigen presenting cells (APC) increases immune responses. However, it is unclear if more than one APC-specific targeting unit in the antigenic molecule will increase responses. To address this issue, we have here made heterodimeric vaccine molecules that each express four different fusion subunits. The bacterial ribonuclease barnase and its inhibitor barstar interact with high affinity, and the barnase-barstar complex was therefore used as a dimerization unit. Barnase and barstar were fused N-terminally with single chain fragment variable (scFv)s targeting units specific for either MHC class II molecules on APC or the hapten 5-iodo-4-hydroxy-3-nitrophenylacetyl (NIP). C-terminal antigenic fusions were either the fluorescent protein mCherry or scFv(315) derived from myeloma protein M315. The heterodimeric vaccine molecules were formed both in vitro and in vivo. Moreover, the four different fused moieties appeared to fold correctly since they retained their specificity and function. DNA vaccination with MHC class II-targeted vaccine induced higher mCherry-specific IgG1 responses compared to non-targeted control. Since mCherry and MHC class II are in trans in this heterodimer, this suggests that heterodimeric proteins are formed in vivo without prior protein purification. Surprisingly, one targeting moiety was sufficient for the increased IgG1 response, and addition of a second targeting moiety did not increase responses. Similar results were found in in vitro T cell assays; vaccine molecules with one targeting unit were as potent as those with two. In combination with the easy cloning strategy, the heterodimeric barnase-barstar vaccine molecule could provide a flexible platform for development of novel DNA vaccines with increased potency." @default.
• W2012312915 created "2016-06-24" @default.
• W2012312915 creator A5024078995 @default.
• W2012312915 creator A5053065066 @default.
• W2012312915 creator A5083802834 @default.
• W2012312915 date "2012-09-18" @default.
• W2012312915 modified "2023-09-27" @default.
• W2012312915 title "Heterodimeric Barnase-Barstar Vaccine Molecules: Influence of One versus Two Targeting Units Specific for Antigen Presenting Cells" @default.
• W2012312915 cites W1493816460 @default.
• W2012312915 cites W1523616589 @default.
• W2012312915 cites W1551760143 @default.
• W2012312915 cites W1594950156 @default.
• W2012312915 cites W1622542255 @default.
• W2012312915 cites W1677285588 @default.
• W2012312915 cites W1767261666 @default.
• W2012312915 cites W1920268203 @default.
• W2012312915 cites W1962461815 @default.
• W2012312915 cites W1965859342 @default.
• W2012312915 cites W1966667477 @default.
• W2012312915 cites W1966670960 @default.
• W2012312915 cites W1971955404 @default.
• W2012312915 cites W1973081965 @default.
• W2012312915 cites W1973966982 @default.
• W2012312915 cites W1985033543 @default.
• W2012312915 cites W1987471547 @default.
• W2012312915 cites W1990459596 @default.
• W2012312915 cites W1991130269 @default.
• W2012312915 cites W1992045336 @default.
• W2012312915 cites W2008434921 @default.
• W2012312915 cites W2011591089 @default.
• W2012312915 cites W2011995348 @default.
• W2012312915 cites W2015750714 @default.
• W2012312915 cites W2022542179 @default.
• W2012312915 cites W2029762187 @default.
• W2012312915 cites W2030780518 @default.
• W2012312915 cites W2033094913 @default.
• W2012312915 cites W2039586626 @default.
• W2012312915 cites W2044809556 @default.
• W2012312915 cites W2051638006 @default.
• W2012312915 cites W2052925324 @default.
• W2012312915 cites W2058808969 @default.
• W2012312915 cites W2066094880 @default.
• W2012312915 cites W2079602362 @default.
• W2012312915 cites W2082016799 @default.
• W2012312915 cites W2082820578 @default.
• W2012312915 cites W2103480481 @default.
• W2012312915 cites W2114926334 @default.
• W2012312915 cites W2115734610 @default.
• W2012312915 cites W2123477089 @default.
• W2012312915 cites W2132929600 @default.
• W2012312915 cites W2143695512 @default.
• W2012312915 cites W2147556670 @default.
• W2012312915 cites W2171356751 @default.
• W2012312915 cites W290550136 @default.
• W2012312915 cites W9592554 @default.
• W2012312915 doi "https://doi.org/10.1371/journal.pone.0045393" @default.
• W2012312915 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3445521" @default.
• W2012312915 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23028981" @default.
• W2012312915 hasPublicationYear "2012" @default.
• W2012312915 type Work @default.
• W2012312915 sameAs 2012312915 @default.
• W2012312915 citedByCount "13" @default.
• W2012312915 countsByYear W20123129152013 @default.
• W2012312915 countsByYear W20123129152014 @default.
• W2012312915 countsByYear W20123129152015 @default.
• W2012312915 countsByYear W20123129152016 @default.
• W2012312915 countsByYear W20123129152019 @default.
• W2012312915 countsByYear W20123129152020 @default.
• W2012312915 countsByYear W20123129152021 @default.
• W2012312915 countsByYear W20123129152022 @default.
• W2012312915 crossrefType "journal-article" @default.
• W2012312915 hasAuthorship W2012312915A5024078995 @default.
• W2012312915 hasAuthorship W2012312915A5053065066 @default.
• W2012312915 hasAuthorship W2012312915A5083802834 @default.
• W2012312915 hasBestOaLocation W20123129151 @default.
• W2012312915 hasConcept C104317684 @default.
• W2012312915 hasConcept C142613039 @default.
• W2012312915 hasConcept C147483822 @default.
• W2012312915 hasConcept C153911025 @default.
• W2012312915 hasConcept C170627219 @default.
• W2012312915 hasConcept C185592680 @default.
• W2012312915 hasConcept C207936829 @default.
• W2012312915 hasConcept C2776559745 @default.
• W2012312915 hasConcept C2778976237 @default.
• W2012312915 hasConcept C2779260011 @default.
• W2012312915 hasConcept C54355233 @default.
• W2012312915 hasConcept C55493867 @default.
• W2012312915 hasConcept C67705224 @default.
• W2012312915 hasConcept C86803240 @default.
• W2012312915 hasConcept C95444343 @default.
• W2012312915 hasConceptScore W2012312915C104317684 @default.
• W2012312915 hasConceptScore W2012312915C142613039 @default.
• W2012312915 hasConceptScore W2012312915C147483822 @default.
• W2012312915 hasConceptScore W2012312915C153911025 @default.
• W2012312915 hasConceptScore W2012312915C170627219 @default.
• W2012312915 hasConceptScore W2012312915C185592680 @default.
• W2012312915 hasConceptScore W2012312915C207936829 @default.
• W2012312915 hasConceptScore W2012312915C2776559745 @default.

• next