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• W2012324888 abstract "Problem Tumor necrosis factor (TNF) and soluble TNF receptors (sTNF‐Rs) system related with Th1 and Th2 and activity of NF‐ κ B/I κ B regulatory system. This study was designed to compare sTNF‐R1 and sTNF‐R2 production (shedding) and levels of late activated CD8 + T‐lymphocytes in non‐pregnant ( n = 30) and pregnant ( n = 20) normal women and non‐pregnant ( n = 20) and pregnant ( n = 30) RSA women. Effects of progesterone (natural structure) injections in RSA women were studied. Methods of study Levels of sTNF‐R1, sTNF‐R2, TNF in peripheral blood serum were detected by enzyme‐linked immunosorbent assay. Lymphocyte subsets were estimated by multicolor flow cytometry. NK cell cytotoxic activity of peripheral blood lymphocytes (PBL) in whole blood against K562 targets was determined using Europium‐release cytotoxicity assay. Mitogen‐induced proliferative response of PBL to PHA‐P, Con A and PWM were determined by standard 3 H‐thymidine incorporation assay. Results Levels of soluble TNF‐R1 and TNF‐R2 in normal pregnancy were elevated when compared with non‐pregnant normal women and pregnant RSA women. Levels of late activated CD8 + T‐lymphocytes in normal pregnancy were decreased but no changes were detected in RSA women. After progesterone therapy (i.m. injections of 2.5% oil solution) in RSA women elevation of sTNF‐R1 and sTNF‐R2 to normal pregnancy ranges was observed. No changes in levels of late activated CD8 + T‐lymphocytes after progesterone treatment were detected. Conclusions Elevation of levels of sTNF‐R1, sTNF‐R2 and decrease of late activated cytotoxic T‐lymphocytes are pronounce markers of normal human pregnancy. In RSA women there are no elevation of sTNF‐R1 and sTNF‐R2 levels during pregnancy. This deficiency may be restored by progesterone treatment." @default.
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• W2012324888 date "2005-10-07" @default.
• W2012324888 modified "2023-10-12" @default.
• W2012324888 title "Lack of Soluble TNF-receptors in Women with Recurrent Spontaneous Abortion and Possibility for its Correction" @default.
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• W2012324888 doi "https://doi.org/10.1111/j.1600-0897.2005.00308.x" @default.
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