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• W2012331119 abstract "Polyhydroxyalkanoates (PHAs), as a candidate for biodegradable plastic materials, can be synthesized by numerous microorganisms. However, as its production cost is high in comparison with those of chemically synthesized plastics, a lot of research has been focused on the efficient production of PHAs using different methods. In the present study, the mutation effects of PHAs production in strain pCB4 were investigated with implantation of low energy ions. It was found that under the implantation conditions of 7.8 × 1014 N+/cm2 at 10 keV, a high-yield PHAs strain with high genetic stability was generated from many mutants. After optimizing its fermentation conditions, the biomass, PHAs concentration and PHAs content of pCBH4 reached 2.26 g/L, 1.81 g/L, and 80.08% respectively, whereas its wild type controls were about 1.24 g/L, 0.61 g/L, and 49.20%. Moreover, the main constituent of PHAs was identified as poly-3-hydroxybutyrates (PHB) in the mutant stain and the yield of this compound was increased up to 41.33% in contrast to that of 27.78% in the wild type strain." @default.
• W2012331119 created "2016-06-24" @default.
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• W2012331119 date "2008-12-01" @default.
• W2012331119 modified "2023-09-26" @default.
• W2012331119 title "Creation of High-Yield Polyhydroxyalkanoates Engineered Strains by Low Energy Ion Implantation" @default.
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• W2012331119 doi "https://doi.org/10.1088/1009-0630/10/6/23" @default.
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