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- W2012334781 abstract "Determination of the internal pH of halobacterial cells grown in 4M salt solution has proven to be a difficult problem. We now report the steady state cytosolic pH of Halobacteriumhalobium S-9 to be 7.2. Intracellular pH was determined after the cells were loaded with the membrane permeable precursor of the pH sensitive dye 2′, 7′-bis-(carboxyethyl)-5(6)-carboxyfluorescein-acetyxymethyl ester — (BCECF/AM). In order to minimize light-scattering in the measurement of the fluorescence, a thin cuvette was newly devised. This method should be suitable for studies of the cytosolic pH in other bacteria." @default.
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- W2012334781 date "1988-08-01" @default.
- W2012334781 modified "2023-10-16" @default.
- W2012334781 title "Intracellular pH of halobacteria can be determined by the fluorescent dye 2′, 7′-bis(carboxyethyl)-5(6)-carboxyfluorescein" @default.
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- W2012334781 doi "https://doi.org/10.1016/s0006-291x(88)81058-1" @default.
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