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• W2012343703 abstract "Human eosinophil cationic protein (ECP) and eosinophil derived neurotoxin (EDN) are two ribonuclease A (RNaseA) family members secreted by activated eosinophils. They share conserved catalytic triad and similar three dimensional structures. ECP and EDN are heparin binding proteins with diverse biological functions. We predicted a novel molecular model for ECP binding of heparin hexasaccharide (Hep6), [GlcNS(6S)-IdoA(2S)]3, and residues Gln40, His64 and Arg105 were indicated as major contributions for the interaction. Interestingly, Gln40 and His64 on ECP formed a clamp-like structure to stabilize Hep6 in our model, which was not observed in the corresponding residues on EDN. To validate our prediction, mutant ECPs including ECP Q40A, H64A, R105A, and double mutant ECP Q40A/H64A were generated, and their binding affinity for heparins were measured by isothermal titration calorimetry (ITC). Weaker binding of ECP Q40A/H64A of all heparin variants suggested that Gln40-His64 clamp contributed to ECP-heparin interaction significantly. Our in silico and in vitro data together demonstrate that ECP uses not only major heparin binding region but also use other surrounding residues to interact with heparin. Such correlation in sequence, structure, and function is a unique feature of only higher primate ECP, but not EDN." @default.
• W2012343703 created "2016-06-24" @default.
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• W2012343703 date "2013-12-11" @default.
• W2012343703 modified "2023-09-24" @default.
• W2012343703 title "Functional Characterization of ECP-Heparin Interaction: A Novel Molecular Model" @default.
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• W2012343703 doi "https://doi.org/10.1371/journal.pone.0082585" @default.
• W2012343703 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3859622" @default.
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