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• W2012351775 abstract "Arabidopsis thaliana was transformed with a Sorghum C4 PEPC cDNA under the control of the 35S promoter. Primary transformants were isolated and classified on a seed PEPC activity basis: A lines (two-fold increase) and B lines (four-fold increase). Primary transformants also expressed the C4 PEPC in leaves (up to a 10-fold increase in activity). In seeds, ectopically expressed PEPC impacted on dry weight and total protein content that were increased by up to 30%. In the stable progeny of A and B lines, the increase in PEPC activity was amplified in seeds; however, a decrease in PEPC content and activity (inversely proportional to seed levels) was observed in maternal tissues (leaves and siliques). This was due to the absence of the exogenous C4 PEPC and a partial decrease in the endogenous C3 PEPC forms (mRNA, protein and activity). There was no apparent compensation by a PEPC hyperphosphorylation. In these plants, the anaplerotic pathway flux was altered (notably, a decrease in organic acids and glutamine levels) as judged by 1H NMR metabolite profiling. Whereas in the corresponding seeds (four- to eight-fold increase in PEPC activity) there was no increase in protein and seed mass, but a moderate decrease in lipid content was observed (compared to control plants). Under normal culture conditions, the transformants (primary and progeny) did not show any apparent growth phenotype or modification in seed production per plant. However, the A and B lines exhibited severe growth defects when salt stressed by LiCl." @default.
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• W2012351775 date "2007-02-01" @default.
• W2012351775 modified "2023-09-25" @default.
• W2012351775 title "Physiological impacts of modulating phosphoenolpyruvate carboxylase levels in leaves and seeds of Arabidopsis thaliana" @default.
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• W2012351775 doi "https://doi.org/10.1016/j.plantsci.2006.09.008" @default.
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