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- W2012364254 abstract "Okadaic acid (OA), a lipophilic phycotoxin is mainly produced by toxigenic dinoflagellates. The need to develop high performing methods for OA analysis able to improve the traditional ones is evident. In this work, a novel experimental methodology for label-free detection of OA was developed. Protein G magnetic beads (protein-G-MBs) modified gold electrode was used to immobilize anti-OA monoclonal antibody (anti-OA-MAb). Preliminary, colorimetric tests were performed in order to validate protein-G-MBs and anti-OA-MAb reaction. Electrochemical detection was carried out by differential pulse voltammetry in ferri/ferrocyanide solution. The limit of detection value obtained (0.5 μg L−1) validated the developed electrochemical immunosensor as a promising tool for routine use. The matrix effect and the recovery rate were also assessed with real samples, showing a good percentage of recovery." @default.
- W2012364254 created "2016-06-24" @default.
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- W2012364254 date "2012-04-01" @default.
- W2012364254 modified "2023-10-07" @default.
- W2012364254 title "Development of a novel label-free amperometric immunosensor for the detection of okadaic acid" @default.
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- W2012364254 doi "https://doi.org/10.1016/j.aca.2012.02.035" @default.
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