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- W2012365250 abstract "Abstract Incubation of salivary glands of Drosophila melanogaster larvae with protein synthesis-inhibiting antibiotics for 3–4 hr in vitro resulted in specific puff induction. From the puffs tested, cycloheximide (100 μg/ml) induced 63E, 47B and 50CD, pactamycin (100 μg/ml) 63E only. Puromycin (100 μg/ml) and fusidic acid (100 μg/ml) on the other hand induced the heat- and 2,4-dinitrophenol-sensitive group of puffs (Ashburner, 1970) when applied to glands of a certain developmental stage (∼117 hr). Puffs 63BC, 87A, 87B, and 93D were measured; the increase in size was, as in the other group, statistically significant. Combined treatment with equal doses of cycloheximide and puromycin lead to cycloheximide-specific puffing pattern only; the same was true for lower doses of cycloheximide (20 μg/ml). 1 μg/ml cycloheximide did not induce any of the puff groups but prevented inductions by puromycin. The kinetics of the puff induction is specific for the different puffs measured. The effect of puromycin could be prevented by adding α-amanitin (1 μg/ml) or by ouabain (10 −4 M), not, however, by adding ATP (10 −3 M). The results may indicate that puffs are regulated via protein repressors and/or activators, the action of which is influenced by energy dependent transport processes." @default.
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- W2012365250 date "1973-05-01" @default.
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- W2012365250 title "The effects of protein synthesis-inhibiting antibiotics on the puffing pattern of Drosophila salivary gland chromosomes in vitro" @default.
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- W2012365250 doi "https://doi.org/10.1016/0045-6039(73)90025-0" @default.
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