Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012448066> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2012448066 endingPage "3178" @default.
- W2012448066 startingPage "3167" @default.
- W2012448066 abstract "The effect of clomiphene, an ovulation-inducing agent, on cytosolic free Ca(2+) levels ([Ca(2+)](i)) in populations of PC3 human prostate cancer cells was explored by using fura-2 as a Ca(2+) indicator. Clomiphene at concentrations between 10-50 microM increased [Ca(2+)](i) in a concentration-dependent manner. The [Ca(2+)](i) signal was biphasic with an initial rise and a slow decay. Ca(2+) removal inhibited the Ca(2+) signal by 41%. Adding 3 mM Ca(2+) increased [Ca(2+)](i) in cells pretreated with clomiphene in Ca(2+)-free medium, confirming that clomiphene induced Ca(2+) entry. In Ca(2+)-free medium, pretreatment with 50 microM brefeldin A (to permeabilize the Golgi complex), 1 microM thapsigargin (to inhibit the endoplasmic reticulum Ca(2+) pump), and 2 microM carbonylcyanide m-chlorophenylhydrazone (to uncouple mitochondria) inhibited 25% of 50 microM clomiphene-induced store Ca(2+) release. Conversely, pretreatment with 50 microM clomiphene in Ca(2+)-free medium abolished the [Ca(2+)](i) increase induced by brefeldin A, thapsigargin or carbonylcyanide m-chlorophenylhydrazone. The 50 microM clomiphene-induced Ca(2+)release was unaltered by inhibiting phospholipase C with 2 microM 1-(6-((17beta-3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione (U73122). Trypan blue exclusion assay suggested that incubation with clomiphene (50 microM) for 2-15 min induced time-dependent decrease in cell viability by 10-50%. Collectively, the results suggest that clomiphene induced [Ca(2+)](i) increases in PC3 cells by releasing store Ca(2+) from multiple stores in an phospholipase C-independent manner, and by activating Ca(2+) influx; and clomiphene was of mild cytotoxicity." @default.
- W2012448066 created "2016-06-24" @default.
- W2012448066 creator A5012700198 @default.
- W2012448066 creator A5027423438 @default.
- W2012448066 creator A5036563270 @default.
- W2012448066 creator A5054056894 @default.
- W2012448066 creator A5058199783 @default.
- W2012448066 date "2002-05-01" @default.
- W2012448066 modified "2023-09-23" @default.
- W2012448066 title "Effect of clomiphene on Ca2+ movement in human prostate cancer cells" @default.
- W2012448066 cites W1574660002 @default.
- W2012448066 cites W1976661289 @default.
- W2012448066 cites W1980166477 @default.
- W2012448066 cites W1992475843 @default.
- W2012448066 cites W2015970432 @default.
- W2012448066 cites W2020165439 @default.
- W2012448066 cites W2034862605 @default.
- W2012448066 cites W2036634370 @default.
- W2012448066 cites W2041256074 @default.
- W2012448066 cites W2042004421 @default.
- W2012448066 cites W2049779407 @default.
- W2012448066 cites W2057665893 @default.
- W2012448066 cites W2060196770 @default.
- W2012448066 cites W2063535351 @default.
- W2012448066 cites W2074508251 @default.
- W2012448066 cites W2086204326 @default.
- W2012448066 cites W2110068210 @default.
- W2012448066 cites W2131184855 @default.
- W2012448066 cites W2141260882 @default.
- W2012448066 cites W2160843895 @default.
- W2012448066 cites W2408884739 @default.
- W2012448066 cites W4242250728 @default.
- W2012448066 doi "https://doi.org/10.1016/s0024-3205(02)01574-6" @default.
- W2012448066 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12008099" @default.
- W2012448066 hasPublicationYear "2002" @default.
- W2012448066 type Work @default.
- W2012448066 sameAs 2012448066 @default.
- W2012448066 citedByCount "13" @default.
- W2012448066 countsByYear W20124480662017 @default.
- W2012448066 crossrefType "journal-article" @default.
- W2012448066 hasAuthorship W2012448066A5012700198 @default.
- W2012448066 hasAuthorship W2012448066A5027423438 @default.
- W2012448066 hasAuthorship W2012448066A5036563270 @default.
- W2012448066 hasAuthorship W2012448066A5054056894 @default.
- W2012448066 hasAuthorship W2012448066A5058199783 @default.
- W2012448066 hasConcept C119062480 @default.
- W2012448066 hasConcept C126322002 @default.
- W2012448066 hasConcept C134018914 @default.
- W2012448066 hasConcept C158617107 @default.
- W2012448066 hasConcept C181199279 @default.
- W2012448066 hasConcept C185592680 @default.
- W2012448066 hasConcept C2777146706 @default.
- W2012448066 hasConcept C2778597717 @default.
- W2012448066 hasConcept C2779965811 @default.
- W2012448066 hasConcept C2781373781 @default.
- W2012448066 hasConcept C28859421 @default.
- W2012448066 hasConcept C55493867 @default.
- W2012448066 hasConcept C62478195 @default.
- W2012448066 hasConcept C71924100 @default.
- W2012448066 hasConcept C86803240 @default.
- W2012448066 hasConcept C98539663 @default.
- W2012448066 hasConceptScore W2012448066C119062480 @default.
- W2012448066 hasConceptScore W2012448066C126322002 @default.
- W2012448066 hasConceptScore W2012448066C134018914 @default.
- W2012448066 hasConceptScore W2012448066C158617107 @default.
- W2012448066 hasConceptScore W2012448066C181199279 @default.
- W2012448066 hasConceptScore W2012448066C185592680 @default.
- W2012448066 hasConceptScore W2012448066C2777146706 @default.
- W2012448066 hasConceptScore W2012448066C2778597717 @default.
- W2012448066 hasConceptScore W2012448066C2779965811 @default.
- W2012448066 hasConceptScore W2012448066C2781373781 @default.
- W2012448066 hasConceptScore W2012448066C28859421 @default.
- W2012448066 hasConceptScore W2012448066C55493867 @default.
- W2012448066 hasConceptScore W2012448066C62478195 @default.
- W2012448066 hasConceptScore W2012448066C71924100 @default.
- W2012448066 hasConceptScore W2012448066C86803240 @default.
- W2012448066 hasConceptScore W2012448066C98539663 @default.
- W2012448066 hasIssue "26" @default.
- W2012448066 hasLocation W20124480661 @default.
- W2012448066 hasLocation W20124480662 @default.
- W2012448066 hasOpenAccess W2012448066 @default.
- W2012448066 hasPrimaryLocation W20124480661 @default.
- W2012448066 hasRelatedWork W1969425564 @default.
- W2012448066 hasRelatedWork W1975315927 @default.
- W2012448066 hasRelatedWork W1979165398 @default.
- W2012448066 hasRelatedWork W1999918524 @default.
- W2012448066 hasRelatedWork W2017283026 @default.
- W2012448066 hasRelatedWork W2075222873 @default.
- W2012448066 hasRelatedWork W2133064571 @default.
- W2012448066 hasRelatedWork W2161342198 @default.
- W2012448066 hasRelatedWork W2437545689 @default.
- W2012448066 hasRelatedWork W2795802867 @default.
- W2012448066 hasVolume "70" @default.
- W2012448066 isParatext "false" @default.
- W2012448066 isRetracted "false" @default.
- W2012448066 magId "2012448066" @default.
- W2012448066 workType "article" @default.