Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012456730> ?p ?o ?g. }
- W2012456730 endingPage "6004" @default.
- W2012456730 startingPage "5998" @default.
- W2012456730 abstract "Mycobacterium bovis bacillus Calmette-Guerin (BCG), the only vaccine currently used against tuberculosis, is an attenuated derivative of M. bovis that has been propagated in vitro for more than 40 years. We have previously reported that the experimentally-verified human T cell epitopes of the M. tuberculosis complex (MTBC) are the most conserved elements of the genome; whether immune recognition is the force driving the conservation of epitopes in the MTBC is unknown. Therefore, we sequenced the genomes of 12 BCG strains to determine whether T cell epitopes were under selection pressure during BCG in vitro evolution. We constructed a genome-wide phylogeny and refined the previously-determined BCG phylogeny. Notably, we identified a new cluster between BCG Japan and BCG Russia, and repositioned the relationships of several strains within the lineage. We also compared the sequence diversity of 1530 experimentally verified human T cell epitopes in the BCG vaccines with those in the MTBC. We found 23% of the known T cell epitopes are absent, and that the majority (82%) of the absent epitopes in BCG are contained in 6 proteins encoded in 2 regions of difference (RD) unique to BCG strains. We also found that T cell epitope sequences in BCG are more conserved than non-epitope sequences in the same gene. Finally, we find evidence that epitope sequence variation in BCG potentially affects human T cell recognition. These findings provide new insight into sequence variation in a slow-growing bacterium closely related to the MTBC that has been subjected to prolonged passage outside of a mammalian host, and indicate little difference in the extent of variation in vivo and in vitro." @default.
- W2012456730 created "2016-06-24" @default.
- W2012456730 creator A5001673483 @default.
- W2012456730 creator A5009537884 @default.
- W2012456730 creator A5053236531 @default.
- W2012456730 creator A5078960996 @default.
- W2012456730 creator A5081756586 @default.
- W2012456730 date "2014-10-01" @default.
- W2012456730 modified "2023-10-15" @default.
- W2012456730 title "Impact of in vitro evolution on antigenic diversity of Mycobacterium bovis bacillus Calmette-Guerin (BCG)" @default.
- W2012456730 cites W1502264478 @default.
- W2012456730 cites W1953458801 @default.
- W2012456730 cites W1966264673 @default.
- W2012456730 cites W1967679421 @default.
- W2012456730 cites W1992070048 @default.
- W2012456730 cites W1993772622 @default.
- W2012456730 cites W1994939019 @default.
- W2012456730 cites W2005360702 @default.
- W2012456730 cites W2024974612 @default.
- W2012456730 cites W2035593347 @default.
- W2012456730 cites W2036218860 @default.
- W2012456730 cites W2039349294 @default.
- W2012456730 cites W2049675690 @default.
- W2012456730 cites W2066321034 @default.
- W2012456730 cites W2067834832 @default.
- W2012456730 cites W2094939159 @default.
- W2012456730 cites W2100531445 @default.
- W2012456730 cites W2100894095 @default.
- W2012456730 cites W2102217401 @default.
- W2012456730 cites W2103230271 @default.
- W2012456730 cites W2105341822 @default.
- W2012456730 cites W2106682613 @default.
- W2012456730 cites W2106814707 @default.
- W2012456730 cites W2119512408 @default.
- W2012456730 cites W2121476879 @default.
- W2012456730 cites W2130362098 @default.
- W2012456730 cites W2131871678 @default.
- W2012456730 cites W2137617609 @default.
- W2012456730 cites W2142347924 @default.
- W2012456730 cites W2157559696 @default.
- W2012456730 cites W2165475706 @default.
- W2012456730 cites W2168688111 @default.
- W2012456730 cites W4205161959 @default.
- W2012456730 doi "https://doi.org/10.1016/j.vaccine.2014.07.113" @default.
- W2012456730 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4539939" @default.
- W2012456730 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25211768" @default.
- W2012456730 hasPublicationYear "2014" @default.
- W2012456730 type Work @default.
- W2012456730 sameAs 2012456730 @default.
- W2012456730 citedByCount "54" @default.
- W2012456730 countsByYear W20124567302014 @default.
- W2012456730 countsByYear W20124567302015 @default.
- W2012456730 countsByYear W20124567302016 @default.
- W2012456730 countsByYear W20124567302017 @default.
- W2012456730 countsByYear W20124567302018 @default.
- W2012456730 countsByYear W20124567302019 @default.
- W2012456730 countsByYear W20124567302020 @default.
- W2012456730 countsByYear W20124567302021 @default.
- W2012456730 countsByYear W20124567302022 @default.
- W2012456730 countsByYear W20124567302023 @default.
- W2012456730 crossrefType "journal-article" @default.
- W2012456730 hasAuthorship W2012456730A5001673483 @default.
- W2012456730 hasAuthorship W2012456730A5009537884 @default.
- W2012456730 hasAuthorship W2012456730A5053236531 @default.
- W2012456730 hasAuthorship W2012456730A5078960996 @default.
- W2012456730 hasAuthorship W2012456730A5081756586 @default.
- W2012456730 hasBestOaLocation W20124567302 @default.
- W2012456730 hasConcept C104317684 @default.
- W2012456730 hasConcept C141231307 @default.
- W2012456730 hasConcept C142724271 @default.
- W2012456730 hasConcept C147483822 @default.
- W2012456730 hasConcept C159047783 @default.
- W2012456730 hasConcept C195616568 @default.
- W2012456730 hasConcept C22070199 @default.
- W2012456730 hasConcept C2777975735 @default.
- W2012456730 hasConcept C2778288313 @default.
- W2012456730 hasConcept C2778497218 @default.
- W2012456730 hasConcept C2779790121 @default.
- W2012456730 hasConcept C2780374374 @default.
- W2012456730 hasConcept C2781069245 @default.
- W2012456730 hasConcept C2781241564 @default.
- W2012456730 hasConcept C523546767 @default.
- W2012456730 hasConcept C54355233 @default.
- W2012456730 hasConcept C71924100 @default.
- W2012456730 hasConcept C86803240 @default.
- W2012456730 hasConcept C89423630 @default.
- W2012456730 hasConceptScore W2012456730C104317684 @default.
- W2012456730 hasConceptScore W2012456730C141231307 @default.
- W2012456730 hasConceptScore W2012456730C142724271 @default.
- W2012456730 hasConceptScore W2012456730C147483822 @default.
- W2012456730 hasConceptScore W2012456730C159047783 @default.
- W2012456730 hasConceptScore W2012456730C195616568 @default.
- W2012456730 hasConceptScore W2012456730C22070199 @default.
- W2012456730 hasConceptScore W2012456730C2777975735 @default.
- W2012456730 hasConceptScore W2012456730C2778288313 @default.
- W2012456730 hasConceptScore W2012456730C2778497218 @default.
- W2012456730 hasConceptScore W2012456730C2779790121 @default.
- W2012456730 hasConceptScore W2012456730C2780374374 @default.