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- W2012483466 abstract "Abstract Antibodies produced against BSA-bromouridine react with bromodeoxyuridine (BUdR) alone, or when incorporated into single-stranded DNA. This was shown by double-diffusion and microcomplement fixation techniques. The antibody, purified by affinity chromatography, can be used for immunofluorescent analysis of DNA replication in single cells, as an alternative to autoradiography with [ 3 H]thymidine. Pulses of BUdR, of 30 min duration, are sufficient for the immunofluorescent detection of DNA synthesis. In addition, the antibody can be used to detect sister chromatid exchanges by the use of the immunoperoxidase technique." @default.
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- W2012483466 date "1975-10-01" @default.
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- W2012483466 title "The use of antibody specific for bromodeoxyuridine for the immunofluorescent determination of DNA replication in single cells and chromosomes" @default.
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- W2012483466 doi "https://doi.org/10.1016/0014-4827(75)90612-6" @default.
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