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- W2012483816 abstract "Abstract The pH optimum for photosynthetic phosphorylation by spinach chloroplasts is found to be 8.0. The reaction can be measured, when run in bicarbonate buffer with carbon dioxide in the atmosphere, as carbon dioxide uptake, due to the pH change as phosphate uptake occurs. An extractable enzymic factor necessary for phosphorylation to proceed has been purified ten-fold by ammonium sulfate precipitation. The extracted enzyme will stimulate phosphorylation by washed or unwashed chloroplasts. In the latter instance, maximal rates of uptake of about 200 μmoles of phosphate/mg chlorophyll/h can be observed. Some implications of this rapid rate of phosphorylation are discussed. Washed chloroplasts will esterify phosphate if the extracted and purified factor is added back. The co-factor requirements in this purified system have been investigated, and are essentially the same as those originally reported." @default.
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- W2012483816 date "1957-11-01" @default.
- W2012483816 modified "2023-09-26" @default.
- W2012483816 title "Photosynthetic phosphorylation in a partially purified system" @default.
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- W2012483816 doi "https://doi.org/10.1016/0006-3002(57)90004-5" @default.
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