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- W2012502775 abstract "Abstract We quantify here, for the first time, the intracellular uptake (J774A.1 murine macrophage cells) of gadolinium‐loaded ultra‐short single‐walled carbon nanotubes (gadonanotubes or GNTs) in a 3 T MRI scanner using R 2 and R 2 * mapping in vitro . GNT‐labeled cells exhibited high and linear changes in net transverse relaxations (Δ R 2 and Δ R ) with increasing cell concentration. The measured Δ R 2 * were about three to four times greater than the respective Δ R 2 for each cell concentration. The intracellular uptake of GNTs was validated with inductively coupled plasma optical emission spectrometry (ICP‐OES), indicating an average cellular uptake of 0.44 ± 0.09 pg Gd per cell or 1.69 × 10 9 Gd 3+ ions per cell. Cell proliferation MTS assays demonstrated that the cells were effectively labeled, without cytotoxicity, for GNTs concentrations ≤28 µ M Gd. In vivo relaxometry of a subcutaneously‐injected GNT‐labeled cell pellet in a mouse was also demonstrated at 3 T. Finally, the pronounced R 2 * effect of GNT‐labeled cells enabled successful in vitro visualization of labeled cells at 9.4 T. Copyright © 2010 John Wiley & Sons, Ltd." @default.
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- W2012502775 date "2010-12-29" @default.
- W2012502775 modified "2023-10-17" @default.
- W2012502775 title "Cellular uptake and imaging studies of gadolinium-loaded single-walled carbon nanotubes as MRI contrast agents" @default.
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- W2012502775 doi "https://doi.org/10.1002/cmmi.410" @default.
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