Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012504248> ?p ?o ?g. }
- W2012504248 endingPage "938" @default.
- W2012504248 startingPage "927" @default.
- W2012504248 abstract "Abstract Gene expression and consequent biological activity of adult tissue stem cells are regulated by signals emanating from the local microenvironment (niche). To gain insights into the molecular regulation of spermatogonial stem cells (SSCs), gene expression was characterized from SSCs isolated from their cognate niches of cryptorchid (stem cell-enriched), wild-type, and busulfan-treated (stem cell-depleted) mouse testes. Quantitative assessment of stem cell activity in each testis model was determined using an in vivo functional assay and correlated with gene expression using Affymetrix MGU74Av2 microarrays and the ChipStat algorithm optimized to detect gene expression from rare cells in complex tissues. We identified 389 stem/progenitor spermatogonia candidate genes, which exhibited significant overlap with genes expressed by embryonic, hematopoietic, and neural stem cells; enriched spermatogonia; and cultured SSCs identified in previous studies. Candidate cell surface markers identified by the microarray may facilitate the isolation and enrichment of stem and/or progenitor spermatogonia. Flow cytometric analyses confirmed the expression of chemokine receptor 2 (Ccr2) and Cd14 on a subpopulation cryptorchid testis cells (α6-integrin+, side scatterlo) enriched for SSCs. These cell surface molecules may mark progenitor spermatogonia but not SSCs because Ccr2+ and Cd14+ fractions failed to produce spermatogenesis upon transplantation to recipient testes. Functional annotation of candidate genes and subsequent immunohistochemistry revealed that proteins involved in post-transcriptional regulation are overrepresented in cryptorchid testes that are enriched for SSCs. Comparative analyses indicated that this is a recurrent biological theme among stem cells. Disclosure of potential conflicts of interest is found at the end of this article." @default.
- W2012504248 created "2016-06-24" @default.
- W2012504248 creator A5018069190 @default.
- W2012504248 creator A5027062877 @default.
- W2012504248 creator A5029054750 @default.
- W2012504248 creator A5063861753 @default.
- W2012504248 creator A5076284560 @default.
- W2012504248 creator A5081184811 @default.
- W2012504248 creator A5085709802 @default.
- W2012504248 creator A5089013559 @default.
- W2012504248 date "2008-01-17" @default.
- W2012504248 modified "2023-09-25" @default.
- W2012504248 title "Genes Involved in Post-Transcriptional Regulation Are Overrepresented in Stem/Progenitor Spermatogonia of Cryptorchid Mouse Testes" @default.
- W2012504248 cites W1499944371 @default.
- W2012504248 cites W1516316082 @default.
- W2012504248 cites W1562641463 @default.
- W2012504248 cites W1572198832 @default.
- W2012504248 cites W1600880434 @default.
- W2012504248 cites W1645470992 @default.
- W2012504248 cites W1969693963 @default.
- W2012504248 cites W1976435237 @default.
- W2012504248 cites W1977825177 @default.
- W2012504248 cites W1978397931 @default.
- W2012504248 cites W1979333438 @default.
- W2012504248 cites W1990205198 @default.
- W2012504248 cites W1990409602 @default.
- W2012504248 cites W1991840668 @default.
- W2012504248 cites W1993491850 @default.
- W2012504248 cites W1997444723 @default.
- W2012504248 cites W1998383188 @default.
- W2012504248 cites W2001627351 @default.
- W2012504248 cites W2014675702 @default.
- W2012504248 cites W2014694767 @default.
- W2012504248 cites W2016789158 @default.
- W2012504248 cites W2021971921 @default.
- W2012504248 cites W2025701349 @default.
- W2012504248 cites W2034488904 @default.
- W2012504248 cites W2038282268 @default.
- W2012504248 cites W2042202436 @default.
- W2012504248 cites W2045847970 @default.
- W2012504248 cites W2048329493 @default.
- W2012504248 cites W2049175933 @default.
- W2012504248 cites W2049964127 @default.
- W2012504248 cites W2057751003 @default.
- W2012504248 cites W2059033739 @default.
- W2012504248 cites W2068464983 @default.
- W2012504248 cites W2070697563 @default.
- W2012504248 cites W2073738799 @default.
- W2012504248 cites W2075977131 @default.
- W2012504248 cites W2083873101 @default.
- W2012504248 cites W2085171607 @default.
- W2012504248 cites W2088612956 @default.
- W2012504248 cites W2090259600 @default.
- W2012504248 cites W2097536533 @default.
- W2012504248 cites W2100089824 @default.
- W2012504248 cites W2101565743 @default.
- W2012504248 cites W2103017472 @default.
- W2012504248 cites W2104359745 @default.
- W2012504248 cites W2104562814 @default.
- W2012504248 cites W2106873615 @default.
- W2012504248 cites W2107794882 @default.
- W2012504248 cites W2110266552 @default.
- W2012504248 cites W2110804359 @default.
- W2012504248 cites W2118327852 @default.
- W2012504248 cites W2118487725 @default.
- W2012504248 cites W2119739306 @default.
- W2012504248 cites W2121877384 @default.
- W2012504248 cites W2127268367 @default.
- W2012504248 cites W2127938920 @default.
- W2012504248 cites W2128880818 @default.
- W2012504248 cites W2131238404 @default.
- W2012504248 cites W2133948311 @default.
- W2012504248 cites W2134097044 @default.
- W2012504248 cites W2138142665 @default.
- W2012504248 cites W2138780833 @default.
- W2012504248 cites W2138910028 @default.
- W2012504248 cites W2140470102 @default.
- W2012504248 cites W2144166281 @default.
- W2012504248 cites W2151201243 @default.
- W2012504248 cites W2151803242 @default.
- W2012504248 cites W2165703224 @default.
- W2012504248 cites W2168436056 @default.
- W2012504248 cites W2170881245 @default.
- W2012504248 cites W2260001467 @default.
- W2012504248 doi "https://doi.org/10.1634/stemcells.2007-0893" @default.
- W2012504248 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4091824" @default.
- W2012504248 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18203673" @default.
- W2012504248 hasPublicationYear "2008" @default.
- W2012504248 type Work @default.
- W2012504248 sameAs 2012504248 @default.
- W2012504248 citedByCount "34" @default.
- W2012504248 countsByYear W20125042482012 @default.
- W2012504248 countsByYear W20125042482013 @default.
- W2012504248 countsByYear W20125042482016 @default.
- W2012504248 countsByYear W20125042482017 @default.
- W2012504248 countsByYear W20125042482018 @default.
- W2012504248 countsByYear W20125042482019 @default.
- W2012504248 countsByYear W20125042482020 @default.