FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012507614> ?p ?o ?g. }

• W2012507614 endingPage "4662" @default.
• W2012507614 startingPage "4646" @default.
• W2012507614 abstract "A major obstacle to obtaining more detailed insights into the diversity of phenotypic and molecular changes occurring in colon cancer cells is the lack of low-passage colon cancer cell lines, which would still closely reflect the phenotype of the colon cancer cells in vivo. Here, we characterize eight novel, low passage number human colon carcinoma cell lines, originating from colorectal cancers extensively characterized in the clinics. All cell lines closely resemble the original tumors with respect to phenotype, markers and detectable genetic changes. Cell morphology and marker expression is highly variable, ranging from fully polarized cells correctly expressing all basolateral epithelial markers, to cells with mesenchymal characteristics and a complete loss of polarity due to delocalization or loss of junction complex proteins. The alterations in phenotype and epithelial marker expression correspond to changes already detectable in the primary tumor in vivo. Seven of the cell lines show chromosomal instability, while one cell line is characterized by microsatellite instability. p53 associated with K-ras mutations were detected in three cell lines. Hitherto non-described E-cadherin mutations were found at both alleles in one cell line whereas in another cell line the E-cadherin protein was down-regulated. A stabilizing beta-catenin mutation (S45F) appears in the same cell line that carried the mutated E-cadherin gene. Six cell lines carried APC mutations, which in five of the lines led to an activated beta-catenin/Tcf/LEF signaling pathway. In accordance with beta-catenin/Tcf/LEF activation, the cell lines show increased migration and invasiveness. Our results show that the characterized, low-passage cell lines mirror the diversity of the individual tumors from which they were derived. Through molecular analyses of these cell lines we demonstrate that tumorgenicity events are much more diverse in human colon cancer than expected, despite the common origin of the tumors from a small patient group with similar tumor grading and clinical prognosis." @default.
• W2012507614 created "2016-06-24" @default.
• W2012507614 creator A5006188950 @default.
• W2012507614 creator A5016575122 @default.
• W2012507614 creator A5023072755 @default.
• W2012507614 creator A5032058761 @default.
• W2012507614 creator A5034693291 @default.
• W2012507614 creator A5053929487 @default.
• W2012507614 creator A5054345533 @default.
• W2012507614 creator A5059384159 @default.
• W2012507614 creator A5089323856 @default.
• W2012507614 date "2002-07-05" @default.
• W2012507614 modified "2023-10-10" @default.
• W2012507614 title "Novel colon cancer cell lines leading to better understanding of the diversity of respective primary cancers" @default.
• W2012507614 cites W1484020898 @default.
• W2012507614 cites W1509850974 @default.
• W2012507614 cites W1544851750 @default.
• W2012507614 cites W1545398558 @default.
• W2012507614 cites W1554815317 @default.
• W2012507614 cites W1666932156 @default.
• W2012507614 cites W1964574104 @default.
• W2012507614 cites W1966241991 @default.
• W2012507614 cites W1967137553 @default.
• W2012507614 cites W1967304777 @default.
• W2012507614 cites W1973800266 @default.
• W2012507614 cites W1976978893 @default.
• W2012507614 cites W1981155714 @default.
• W2012507614 cites W1982793182 @default.
• W2012507614 cites W1989896581 @default.
• W2012507614 cites W1990085600 @default.
• W2012507614 cites W1991932352 @default.
• W2012507614 cites W1992595726 @default.
• W2012507614 cites W2002404925 @default.
• W2012507614 cites W2005088732 @default.
• W2012507614 cites W2019916351 @default.
• W2012507614 cites W2023821389 @default.
• W2012507614 cites W2024831995 @default.
• W2012507614 cites W2027345413 @default.
• W2012507614 cites W2029848748 @default.
• W2012507614 cites W2029857102 @default.
• W2012507614 cites W2030435075 @default.
• W2012507614 cites W2034030391 @default.
• W2012507614 cites W2034269086 @default.
• W2012507614 cites W2043797012 @default.
• W2012507614 cites W2052870426 @default.
• W2012507614 cites W2053758478 @default.
• W2012507614 cites W2055437294 @default.
• W2012507614 cites W2060937867 @default.
• W2012507614 cites W2065049758 @default.
• W2012507614 cites W2065144976 @default.
• W2012507614 cites W2065990843 @default.
• W2012507614 cites W2071248704 @default.
• W2012507614 cites W2075438156 @default.
• W2012507614 cites W2078981214 @default.
• W2012507614 cites W2081427703 @default.
• W2012507614 cites W2087367170 @default.
• W2012507614 cites W2093531978 @default.
• W2012507614 cites W2093960040 @default.
• W2012507614 cites W2094784381 @default.
• W2012507614 cites W2096053688 @default.
• W2012507614 cites W2096383691 @default.
• W2012507614 cites W2097774235 @default.
• W2012507614 cites W2097829282 @default.
• W2012507614 cites W2100070586 @default.
• W2012507614 cites W2108435741 @default.
• W2012507614 cites W2109069210 @default.
• W2012507614 cites W2111021104 @default.
• W2012507614 cites W2111914361 @default.
• W2012507614 cites W2112420045 @default.
• W2012507614 cites W2123206370 @default.
• W2012507614 cites W2129860749 @default.
• W2012507614 cites W2146173859 @default.
• W2012507614 cites W2149255929 @default.
• W2012507614 cites W2162701366 @default.
• W2012507614 cites W2165901622 @default.
• W2012507614 cites W2167630842 @default.
• W2012507614 cites W2171080110 @default.
• W2012507614 cites W2182875022 @default.
• W2012507614 cites W4229630392 @default.
• W2012507614 cites W4248449407 @default.
• W2012507614 doi "https://doi.org/10.1038/sj.onc.1205577" @default.
• W2012507614 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12096341" @default.
• W2012507614 hasPublicationYear "2002" @default.
• W2012507614 type Work @default.
• W2012507614 sameAs 2012507614 @default.
• W2012507614 citedByCount "56" @default.
• W2012507614 countsByYear W20125076142012 @default.
• W2012507614 countsByYear W20125076142013 @default.
• W2012507614 countsByYear W20125076142014 @default.
• W2012507614 countsByYear W20125076142015 @default.
• W2012507614 countsByYear W20125076142017 @default.
• W2012507614 countsByYear W20125076142018 @default.
• W2012507614 countsByYear W20125076142020 @default.
• W2012507614 countsByYear W20125076142021 @default.
• W2012507614 countsByYear W20125076142023 @default.
• W2012507614 crossrefType "journal-article" @default.
• W2012507614 hasAuthorship W2012507614A5006188950 @default.
• W2012507614 hasAuthorship W2012507614A5016575122 @default.

• next