Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012561030> ?p ?o ?g. }
- W2012561030 endingPage "6438" @default.
- W2012561030 startingPage "6433" @default.
- W2012561030 abstract "Proteins are very sensitive to their solvent environments. Urea is a common chemical denaturant of proteins, yet some animals contain high concentrations of urea. These animals have evolved an interesting mechanism to counteract the effects of urea by using trimethylamine N-oxide (TMAO). The molecular basis for the ability of TMAO to act as a chemical chaperone remains unknown. Here, we describe molecular dynamics simulations of a small globular protein, chymotrypsin inhibitor 2, in 8 M urea and 4 M TMAO/8 M urea solutions, in addition to other control simulations, to investigate this effect at the atomic level. In 8 M urea, the protein unfolds, and urea acts in both a direct and indirect manner to achieve this effect. In contrast, introduction of 4 M TMAO counteracts the effect of urea and the protein remains well structured. TMAO makes few direct interactions with the protein. Instead, it prevents unfolding of the protein by structuring the solvent. In particular, TMAO orders the solvent and discourages it from competing with intraprotein H bonds and breaking up the hydrophobic core of the protein." @default.
- W2012561030 created "2016-06-24" @default.
- W2012561030 creator A5021239442 @default.
- W2012561030 creator A5038376296 @default.
- W2012561030 date "2004-04-19" @default.
- W2012561030 modified "2023-10-09" @default.
- W2012561030 title "Counteraction of urea-induced protein denaturation by trimethylamine <i>N</i> -oxide: A chemical chaperone at atomic resolution" @default.
- W2012561030 cites W1492547275 @default.
- W2012561030 cites W1572204111 @default.
- W2012561030 cites W1576271719 @default.
- W2012561030 cites W1879220584 @default.
- W2012561030 cites W1954489329 @default.
- W2012561030 cites W1970649467 @default.
- W2012561030 cites W1976179026 @default.
- W2012561030 cites W1978033776 @default.
- W2012561030 cites W1983271858 @default.
- W2012561030 cites W1985015433 @default.
- W2012561030 cites W1985107466 @default.
- W2012561030 cites W1986115510 @default.
- W2012561030 cites W1994546561 @default.
- W2012561030 cites W1998641298 @default.
- W2012561030 cites W2011384774 @default.
- W2012561030 cites W2014166395 @default.
- W2012561030 cites W2020563037 @default.
- W2012561030 cites W2023159932 @default.
- W2012561030 cites W2024683933 @default.
- W2012561030 cites W2031215694 @default.
- W2012561030 cites W2032588108 @default.
- W2012561030 cites W2041657602 @default.
- W2012561030 cites W2042964527 @default.
- W2012561030 cites W2047551201 @default.
- W2012561030 cites W2051606057 @default.
- W2012561030 cites W2051726265 @default.
- W2012561030 cites W2056446854 @default.
- W2012561030 cites W2063656283 @default.
- W2012561030 cites W2071545816 @default.
- W2012561030 cites W2075699213 @default.
- W2012561030 cites W2076452226 @default.
- W2012561030 cites W2087579391 @default.
- W2012561030 cites W2089417829 @default.
- W2012561030 cites W2093909695 @default.
- W2012561030 cites W2103208396 @default.
- W2012561030 cites W2106043217 @default.
- W2012561030 cites W2107261781 @default.
- W2012561030 cites W2116512250 @default.
- W2012561030 cites W2132249517 @default.
- W2012561030 cites W2143416345 @default.
- W2012561030 cites W2156084923 @default.
- W2012561030 cites W2160014344 @default.
- W2012561030 cites W2186974610 @default.
- W2012561030 cites W2208224617 @default.
- W2012561030 cites W2283603098 @default.
- W2012561030 cites W2301246770 @default.
- W2012561030 cites W4248782386 @default.
- W2012561030 doi "https://doi.org/10.1073/pnas.0308633101" @default.
- W2012561030 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/404062" @default.
- W2012561030 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15096583" @default.
- W2012561030 hasPublicationYear "2004" @default.
- W2012561030 type Work @default.
- W2012561030 sameAs 2012561030 @default.
- W2012561030 citedByCount "292" @default.
- W2012561030 countsByYear W20125610302012 @default.
- W2012561030 countsByYear W20125610302013 @default.
- W2012561030 countsByYear W20125610302014 @default.
- W2012561030 countsByYear W20125610302015 @default.
- W2012561030 countsByYear W20125610302016 @default.
- W2012561030 countsByYear W20125610302017 @default.
- W2012561030 countsByYear W20125610302018 @default.
- W2012561030 countsByYear W20125610302019 @default.
- W2012561030 countsByYear W20125610302020 @default.
- W2012561030 countsByYear W20125610302021 @default.
- W2012561030 countsByYear W20125610302022 @default.
- W2012561030 countsByYear W20125610302023 @default.
- W2012561030 crossrefType "journal-article" @default.
- W2012561030 hasAuthorship W2012561030A5021239442 @default.
- W2012561030 hasAuthorship W2012561030A5038376296 @default.
- W2012561030 hasBestOaLocation W20125610301 @default.
- W2012561030 hasConcept C105168689 @default.
- W2012561030 hasConcept C12554922 @default.
- W2012561030 hasConcept C13965031 @default.
- W2012561030 hasConcept C147597530 @default.
- W2012561030 hasConcept C185592680 @default.
- W2012561030 hasConcept C2776149385 @default.
- W2012561030 hasConcept C2776923230 @default.
- W2012561030 hasConcept C2780365088 @default.
- W2012561030 hasConcept C2780471494 @default.
- W2012561030 hasConcept C2780924926 @default.
- W2012561030 hasConcept C47701112 @default.
- W2012561030 hasConcept C55493867 @default.
- W2012561030 hasConcept C59593255 @default.
- W2012561030 hasConcept C8010536 @default.
- W2012561030 hasConcept C86803240 @default.
- W2012561030 hasConceptScore W2012561030C105168689 @default.
- W2012561030 hasConceptScore W2012561030C12554922 @default.
- W2012561030 hasConceptScore W2012561030C13965031 @default.
- W2012561030 hasConceptScore W2012561030C147597530 @default.
- W2012561030 hasConceptScore W2012561030C185592680 @default.
- W2012561030 hasConceptScore W2012561030C2776149385 @default.