FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012601256> ?p ?o ?g. }

• W2012601256 endingPage "e84704" @default.
• W2012601256 startingPage "e84704" @default.
• W2012601256 abstract "Neutrophils play a crucial role in host defence. In response to a variety of inflammatory stimulation, they form neutrophil extracellular traps (NETs). NETs are extracellular structures composed of chromatin fibers decorated with antimicrobial proteins and developing studies indicate that NETs contribute to extracellular microbial killing. While the intracellular signaling pathways that regulate NET formation remain largely unknown, there is growing evidence that generation of reactive oxygen species (ROS) is a key event for NET formation. The Rab family small GTPase Rab27a is an important component of the secretory machinery of azurophilic granules in neutrophils. However, the precise mechanism of NET formation and whether or not Rab27a contributes to this process are unknown. Using neutrophil-like differentiated HL60 cells, we show here that Rab27a plays an essential role in both phorbol myristate acetate (PMA)- and Candida albicans-induced NET formation by regulating ROS production. Rab27a-knockdown inhibited ROS-positive phagosome formation during complement-mediated phagocytosis. To investigate the role of Rab27a in neutrophil function in detail, both primary human neutrophils and neutrophil-like differentiated HL60 cells were treated with PMA, and NET formation process was assessed by measurement of release of histone H3 into the medium, citrullination of the arginine in position 3 of histone H4 and chase of the nuclear change of the living cells in the co-existence of both cell-permeable and -impermeable nuclear indicators. PMA-induced NET formation occured sequentially in both neutrophil-like differentiated HL60 cells and primary neutrophils, and Rab27a-knockdown clearly inhibited NET formation in association with reduced ROS production. We also found that serum-treated Candida albicans triggers NET formation in a ROS-dependent manner, and that Rab27a-knockdown inhibits this process as well. Our findings demonstrate that Rab27a plays an important role in NET formation induced by both Candida albicans infection and PMA treatment by regulating ROS production." @default.
• W2012601256 created "2016-06-24" @default.
• W2012601256 creator A5017442070 @default.
• W2012601256 creator A5025137344 @default.
• W2012601256 creator A5029172602 @default.
• W2012601256 creator A5040636868 @default.
• W2012601256 creator A5042193922 @default.
• W2012601256 creator A5058401601 @default.
• W2012601256 creator A5059412860 @default.
• W2012601256 creator A5067043125 @default.
• W2012601256 creator A5085817991 @default.
• W2012601256 date "2014-01-03" @default.
• W2012601256 modified "2023-10-16" @default.
• W2012601256 title "Rab27a Is Essential for the Formation of Neutrophil Extracellular Traps (NETs) in Neutrophil-Like Differentiated HL60 Cells" @default.
• W2012601256 cites W1489286522 @default.
• W2012601256 cites W1675847437 @default.
• W2012601256 cites W1969729143 @default.
• W2012601256 cites W1970320388 @default.
• W2012601256 cites W1973101321 @default.
• W2012601256 cites W1973362572 @default.
• W2012601256 cites W1975005333 @default.
• W2012601256 cites W1979751563 @default.
• W2012601256 cites W1992270172 @default.
• W2012601256 cites W1994668150 @default.
• W2012601256 cites W1994836404 @default.
• W2012601256 cites W1998199040 @default.
• W2012601256 cites W2007626593 @default.
• W2012601256 cites W2011491918 @default.
• W2012601256 cites W2014776493 @default.
• W2012601256 cites W2015828287 @default.
• W2012601256 cites W2016385216 @default.
• W2012601256 cites W2030193400 @default.
• W2012601256 cites W2037265282 @default.
• W2012601256 cites W2041912799 @default.
• W2012601256 cites W2043756611 @default.
• W2012601256 cites W2047012452 @default.
• W2012601256 cites W2067022007 @default.
• W2012601256 cites W2067235043 @default.
• W2012601256 cites W2073676586 @default.
• W2012601256 cites W2096723029 @default.
• W2012601256 cites W2097081315 @default.
• W2012601256 cites W2099356583 @default.
• W2012601256 cites W2099923896 @default.
• W2012601256 cites W2104934425 @default.
• W2012601256 cites W2112543741 @default.
• W2012601256 cites W2115959782 @default.
• W2012601256 cites W2116274871 @default.
• W2012601256 cites W2126774943 @default.
• W2012601256 cites W2132656979 @default.
• W2012601256 cites W2135849676 @default.
• W2012601256 cites W2149832954 @default.
• W2012601256 cites W2150213378 @default.
• W2012601256 cites W2168176302 @default.
• W2012601256 doi "https://doi.org/10.1371/journal.pone.0084704" @default.
• W2012601256 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3880328" @default.
• W2012601256 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24404184" @default.
• W2012601256 hasPublicationYear "2014" @default.
• W2012601256 type Work @default.
• W2012601256 sameAs 2012601256 @default.
• W2012601256 citedByCount "34" @default.
• W2012601256 countsByYear W20126012562014 @default.
• W2012601256 countsByYear W20126012562015 @default.
• W2012601256 countsByYear W20126012562016 @default.
• W2012601256 countsByYear W20126012562017 @default.
• W2012601256 countsByYear W20126012562018 @default.
• W2012601256 countsByYear W20126012562019 @default.
• W2012601256 countsByYear W20126012562020 @default.
• W2012601256 countsByYear W20126012562021 @default.
• W2012601256 countsByYear W20126012562022 @default.
• W2012601256 countsByYear W20126012562023 @default.
• W2012601256 crossrefType "journal-article" @default.
• W2012601256 hasAuthorship W2012601256A5017442070 @default.
• W2012601256 hasAuthorship W2012601256A5025137344 @default.
• W2012601256 hasAuthorship W2012601256A5029172602 @default.
• W2012601256 hasAuthorship W2012601256A5040636868 @default.
• W2012601256 hasAuthorship W2012601256A5042193922 @default.
• W2012601256 hasAuthorship W2012601256A5058401601 @default.
• W2012601256 hasAuthorship W2012601256A5059412860 @default.
• W2012601256 hasAuthorship W2012601256A5067043125 @default.
• W2012601256 hasAuthorship W2012601256A5085817991 @default.
• W2012601256 hasBestOaLocation W20126012561 @default.
• W2012601256 hasConcept C134897094 @default.
• W2012601256 hasConcept C161478664 @default.
• W2012601256 hasConcept C185592680 @default.
• W2012601256 hasConcept C203014093 @default.
• W2012601256 hasConcept C2776914184 @default.
• W2012601256 hasConcept C28406088 @default.
• W2012601256 hasConcept C79879829 @default.
• W2012601256 hasConcept C86803240 @default.
• W2012601256 hasConcept C95444343 @default.
• W2012601256 hasConceptScore W2012601256C134897094 @default.
• W2012601256 hasConceptScore W2012601256C161478664 @default.
• W2012601256 hasConceptScore W2012601256C185592680 @default.
• W2012601256 hasConceptScore W2012601256C203014093 @default.
• W2012601256 hasConceptScore W2012601256C2776914184 @default.
• W2012601256 hasConceptScore W2012601256C28406088 @default.
• W2012601256 hasConceptScore W2012601256C79879829 @default.
• W2012601256 hasConceptScore W2012601256C86803240 @default.

• next