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• W2012601995 abstract "To evaluate the effects of oxidative stress and antioxidants on proliferation of endometrial stromal cells.In vitro study.Academic laboratory.Women, with and without endometriosis, of reproductive age.Culture of endometrial stromal cells with antioxidants or with agents inducing oxidative stress.Proliferation of endometrial stromal cells as determined by thymidine incorporation assay and 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) assay.Antioxidants induced a dose-dependent inhibition of thymidine incorporation: vitamin E succinate was inhibitory at 10-100 microM (by 43%-95%), ebselen at 10-30 microM (by 29%-77%), and N-acetylcysteine at 10-30 mM (by 52%-85%). In contrast, modest oxidative stress induced by hypoxanthine/xanthine oxidase (1 mM/3-30 microU/mL) stimulated proliferation by 40%-62%. H2O2 (1 microM) increased DNA synthesis by 56%. Comparable findings were obtained using MTT proliferation assay. Antioxidants inhibited proliferation: vitamin E succinate (100 microM) by 91%, ebselen (30 microM) by 81%, and N-acetylcysteine (30 mM) by 95%. Hypoxanthine/xanthine oxidase (1 mM/30 microU/mL) and H2O2 (1 microM) stimulated growth by 122% and 58%, respectively.Reactive oxygen species may modulate growth of endometrial stroma. Under pathologic conditions such as endometriosis, increased oxidative stress and depletion of antioxidants may contribute to excessive growth of endometrial stromal cells." @default.
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• W2012601995 date "2004-10-01" @default.
• W2012601995 modified "2023-10-11" @default.
• W2012601995 title "Effects of oxidants and antioxidants on proliferation of endometrial stromal cells" @default.
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• W2012601995 doi "https://doi.org/10.1016/j.fertnstert.2004.02.133" @default.
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