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- W2012603937 abstract "Abstract Viral specific RNA-containing structures released from gently disrupted Sendai virus infected chick embryo fibroblasts were separated into four rapidly sedimenting components in sucrose density gradients. Uridine- 3 H was rapidly incorporated into the RNA of each component. Component 1 (>350 S) contained virion RNA (57 S) and had physical characteristics of cell-associated virus. Component 2 (250 S) contained double-stranded RNA and the kinetics of uridine- 3 H incorporation suggested that this structure may be involved in viral RNA synthesis. Component 3 (200 S) contained 57 S RNA and was identified as viral nucleocapsid. Component 4 (150 S) contained 35 S single-stranded RNA, and within this structure the RNA was protected from RNase digestion. The exact structure of this particle and its role in virus replication must be determined. The RNA from each intracellular component annealed specifically with virion RNA. The 57 S RNAs isolated from the cell-associated virus (component 1) and from the intracellular nucleocapsid (component 3) self annealed as previously shown for virion RNA, and the annealing was RNA concentration dependent. The 57 S RNA from virions could not be separated into more than one component by equilibrium CsCl·Cs 2 SO 4 centrifugation." @default.
- W2012603937 created "2016-06-24" @default.
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- W2012603937 date "1971-01-01" @default.
- W2012603937 modified "2023-09-27" @default.
- W2012603937 title "Intracellular structures involved in sendai virus replication" @default.
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- W2012603937 doi "https://doi.org/10.1016/0042-6822(71)90227-3" @default.
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